Department of Engineering Fundamental Sciences, Tarsus University, Faculty of Engineering, Tarsus, 33400, Turkey.
Department of Environmental Engineering, Mersin University, Mersin, 33343, Turkey.
Chemosphere. 2023 Oct;339:139340. doi: 10.1016/j.chemosphere.2023.139340. Epub 2023 Jun 26.
Antimicrobial resistance to antibiotics for current bacterial infection treatments is a medical problem. 2D nanoparticles, which can be used as both antibiotic carriers and direct antibacterial agents due to their large surface areas and direct contact with the cell membrane, are important alternatives in solving this problem. This study focuses on the effects of a new generation borophene derivative obtained from MgB particles on the antimicrobial activity of polyethersulfone membranes. MgB nanosheets were created by mechanically separating magnesium diboride (MgB) particles into layers. The samples were microstructurally characterized using SEM, HR-TEM, and XRD methods. MgB nanosheets were screened for various biological activities such as antioxidant, DNA nuclease, antimicrobial, microbial cell viability inhibition, and antibiofilm activities. The antioxidant activity of nanosheets was 75.24 ± 4.15% at 200 mg/L. Plasmid DNA was entirely degraded at 125 and 250 mg/L nanosheet concentrations. MgB nanosheets exhibited a potential antimicrobial effect against tested strains. The cell viability inhibitory effect of the MgB nanosheets was 99.7 ± 5.78%, 99.89 ± 6.02%, and 100 ± 5.84% at 12.5 mg/L, 25 mg/L, and 50 mg/L, respectively. The antibiofilm activity of MgB nanosheets against S. aureus and P. aeruginosa was observed to be satisfactory. Furthermore, a polyethersulfone (PES) membrane was prepared by blending MgB nanosheets from 0.5 wt to 2.0 wt %. Pristine PES membrane also has shown the lowest steady-state fluxes at 30.1 ± 2.1 and 56.6 L/mh for BSA and E. coli, respectively. With the increase of MgB nanosheets amount from 0.5 to 2.0 wt%, steady-state fluxes increased from 32.3 ± 2.5 to 42.0 ± 1.0 and from 15.6 ± 0.7 to 24.1 ± 0.8 L/mh, respectively for BSA and E. coli. E. coli elimination performance of PES membrane coated with MgB nanosheets at different rates and the membrane filtration procedure was obtained from 96% to 100%. The results depicted that BSA and E. coli rejection efficiencies of MgB nanosheets blended PES membranes increased when compared to pristine PES membranes.
当前，抗生素治疗细菌性感染的耐药性是一个医学问题。由于二维纳米粒子具有较大的表面积和与细胞膜的直接接触，因此可作为抗生素载体和直接抗菌剂，是解决这一问题的重要选择。本研究关注的是从 MgB 颗粒获得的新一代硼烯衍生物对聚醚砜膜抗菌活性的影响。MgB 纳米片是通过机械分离二硼化镁 (MgB) 颗粒成层而制成的。使用 SEM、HR-TEM 和 XRD 方法对样品进行了微观结构表征。MgB 纳米片具有抗氧化、DNA 核酸酶、抗菌、微生物细胞活力抑制和抗生物膜活性等多种生物学活性。纳米片的抗氧化活性在 200mg/L 时为 75.24±4.15%。在 125 和 250mg/L 纳米片浓度下，质粒 DNA 完全降解。MgB 纳米片对测试菌株表现出潜在的抗菌作用。MgB 纳米片对细胞活力的抑制作用分别为 12.5mg/L 时 99.7±5.78%、25mg/L 时 99.89±6.02%和 50mg/L 时 100±5.84%。MgB 纳米片对金黄色葡萄球菌和铜绿假单胞菌的抗生物膜活性令人满意。此外，通过将 MgB 纳米片从 0.5wt%到 2.0wt%共混到聚醚砜 (PES) 膜中制备了 PES 膜。原始 PES 膜对 BSA 和大肠杆菌的稳态通量也分别显示出最低值，分别为 30.1±2.1 和 56.6L/mh。随着 MgB 纳米片用量从 0.5 增加到 2.0wt%，对 BSA 和大肠杆菌的稳态通量分别从 32.3±2.5 增加到 42.0±1.0 和从 15.6±0.7 增加到 24.1±0.8L/mh。在不同速率下，用 MgB 纳米片涂覆的 PES 膜对 E. coli 的去除性能和膜过滤过程的结果从 96%提高到 100%。结果表明，与原始 PES 膜相比，MgB 纳米片共混的 PES 膜对 BSA 和大肠杆菌的排斥效率增加。
