National Institute of Materials Physics, Street Atomistilor 405A, 077125, Magurele, Romania.
University Politehnica of Bucharest, Splaiul Independentei 313, 060042, Bucharest, Romania.
Sci Rep. 2021 May 5;11(1):9591. doi: 10.1038/s41598-021-88885-2.
Three commercial powders of MgB were tested in vitro by MTS and LDH cytotoxicity tests on the HS27 dermal cell line. Depending on powders, the toxicity concentrations were established in the range of 8.3-33.2 µg/ml. The powder with the lowest toxicity limit was embedded into polyvinylpyrrolidone (PVP), a biocompatible and biodegradable polymer, for two different concentrations. The self-replenishing MgB-PVP composite materials were coated on substrate materials (plastic foil of the reservoir and silicon tubes) composing a commercial urinary catheter. The influence of the PVP-reference and MgB-PVP novel coatings on the bacterial growth of Staphylococcus aureus ATCC 25923, Enterococcus faecium DMS 13590, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, in planktonic and biofilm state was assessed in vitro at 6, 24, and 48 h of incubation time. The MgB-PVP coatings are efficient both against planktonic microbes and microbial biofilms. Results open promising applications for the use of MgB in the design of anti-infective strategies for different biomedical devices and systems.
三种商用 MgB 粉末通过 MTS 和 LDH 细胞毒性试验在 HS27 皮肤细胞系上进行了体外测试。根据粉末的不同，毒性浓度范围在 8.3-33.2μg/ml 之间。毒性最低的粉末被嵌入到聚维酮（PVP）中，PVP 是一种生物相容性和可生物降解的聚合物，用于两种不同的浓度。自补充 MgB-PVP 复合材料被涂覆在基质材料（储液器的塑料箔和硅管）上，构成商业导尿管。PVP 参比材料和 MgB-PVP 新型涂层对金黄色葡萄球菌 ATCC 25923、屎肠球菌 DMS 13590、大肠杆菌 ATCC 25922、铜绿假单胞菌 ATCC 27853 的浮游和生物膜状态下细菌生长的影响在 6、24 和 48 小时的孵育时间内进行了体外评估。MgB-PVP 涂层对浮游微生物和微生物生物膜都有效。这些结果为 MgB 在不同的生物医学设备和系统的抗感染策略设计中的应用提供了有前景的应用。
