Yao Chi-Bei, Feng Lin, Wu Pei, Liu Yang, Jiang Jun, Zhang Lu, Mi Hai-Feng, Zhou Xiao-Qiu, Jiang Wei-Dan
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China.
Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.
Food Chem X. 2023 Jun 10;19:100752. doi: 10.1016/j.fochx.2023.100752. eCollection 2023 Oct 30.
The n6/n3 ratios improved meat quality of terrestrial animals, but alpha-linolenic acid/linoleic acid (ALA/LNA) ratios were rarely studied in aquatic animals. In this study, sub-adult grass carp were fed diets fed diets containing six varying ALA/LNA ratios (0.03, 0.47, 0.92, 1.33, 1.69, and 2.15) for 9 weeks and the total value of n3 + n6 (1.98) was kept constant for all six treatments. The results indicated optimal ALA/LNA ratio improved growth performance, changed fatty acid composition in grass carp muscle, and promoted glucose metabolism. Additionally, optimal ALA/LNA ratio improved chemical attributes by increasing crude protein and lipid contents, and technological attributes by increasing pH value and shear force in grass carp muscle. The signaling pathways related to fatty acid metabolism and glucose metabolism (LXRα/SREBP-1, PPARα, PPARγ, AMPK) might be responsible for these changes. Dietary optimal ALA/LNA ratio based on PWG, UFA and glucose contents was 1.03, 0.88 and 0.92, respectively.
n6/n3 比值改善了陆生动物的肉质,但水生动物中α-亚麻酸/亚油酸(ALA/LNA)比值的研究较少。在本研究中,将亚成年草鱼投喂含有六种不同ALA/LNA 比值(0.03、0.47、0.92、1.33、1.69 和 2.15)的饲料 9 周,且所有六种处理中 n3 + n6 的总值(1.98)保持恒定。结果表明,最佳 ALA/LNA 比值改善了草鱼的生长性能,改变了草鱼肌肉中的脂肪酸组成,并促进了葡萄糖代谢。此外,最佳 ALA/LNA 比值通过增加草鱼肌肉中的粗蛋白和脂质含量改善了化学特性,并通过提高 pH 值和剪切力改善了工艺特性。与脂肪酸代谢和葡萄糖代谢相关的信号通路(LXRα/SREBP-1、PPARα、PPARγ、AMPK)可能是这些变化的原因。基于增重率、不饱和脂肪酸和葡萄糖含量的日粮最佳 ALA/LNA 比值分别为 1.03、0.88 和 0.92。