Forslind B, Li H K, Malmqvist K G, Wiegleb D
Scan Electron Microsc. 1986(Pt 1):237-41.
The elemental content of anagen hair fibers in a Caucasian population of healthy females and males in the age range 10-69 years was performed to constitute a baseline for further studies of pathological conditions. Proton induced X-ray emission (PIXE) analyses were performed on single hair fibers in triplicate from 103 individuals in order to determine sulfur, zinc, calcium, and chlorine content. The hair fibers were all anagen hairs collected from a site little influenced by genetic and hormonal influences 1.5 cm above the right ear of the probands. An area 5-8 mm from the follicle bottom was chosen for the analysis in all cases to minimize effect of hair-do contamination. The average sulfur content was 0.049 g/g and the average zinc content 170 micrograms/g. These results were not significantly influenced by chloroform/ethanol rinsing before analysis. The calcium and chlorine contents were 330 micrograms/g and 0.0033 g/g respectively. The latter data are expected to be more seriously influenced by external factors (e.g., contamination) than sulfur and zinc. No correlation between elemental concentration and sex was found for sulfur and zinc in the present material. PIXE analysis of single hair fibers yields valuable information on the elemental composition of hair fibers and can be rapidly and efficiently performed after simple mounting procedures.
对年龄在10至69岁的高加索健康女性和男性群体的生长期毛发纤维进行元素含量分析，以便为进一步研究病理状况建立基线。对103名个体的单根毛发纤维进行了三次质子诱导X射线发射（PIXE）分析，以确定硫、锌、钙和氯的含量。毛发纤维均为生长期毛发，从先证者右耳上方1.5厘米处采集，该部位受遗传和激素影响较小。在所有情况下，均选择距毛囊底部5至8毫米的区域进行分析，以尽量减少发型污染的影响。平均硫含量为0.049克/克，平均锌含量为170微克/克。分析前用氯仿/乙醇冲洗对这些结果没有显著影响。钙和氯的含量分别为330微克/克和0.0033克/克。预计后一组数据比硫和锌更容易受到外部因素（如污染）的影响。在本研究材料中，未发现硫和锌的元素浓度与性别之间存在相关性。单根毛发纤维的PIXE分析可提供有关毛发纤维元素组成的有价值信息，并且在简单的固定程序后即可快速有效地进行。
