CQM-Centro de Química da Madeira, Universidade da Madeira, Campus da Penteada, 9020-105 Funchal, Portugal.
Advanced (magnetic) theranostic nanostructures lab (AmTheNa), Nanomedicine group, International Iberian Nanotechnology Laboratory (INL), Braga 4715-330, Portugal.
Biomater Adv. 2023 Oct;153:213535. doi: 10.1016/j.bioadv.2023.213535. Epub 2023 Jun 21.
The present work aims to prepare copper-doped MgO nanoparticles via a sol-gel approach and study their antidiabetic alpha-amylase inhibition activity with undoped MgO nanoparticles. The ability of G5 amine-terminated polyamidoamine (PAMAM) dendrimer for the controlled release of copper-doped MgO nanoparticles to exhibit alpha-amylase inhibition activity was also evaluated. The synthesis of MgO nanoparticles via sol-gel approach and optimization of calcination temperature and time has led to the formation of nanoparticles with different shapes (spherical, hexagonal, and rod-shaped) and a polydispersity in size ranging from 10 to 100 nm with periclase crystalline phase. The presence of copper ions in the MgO nanoparticles has altered their crystallite size, eventually modifying their size, morphology, and surface charge. The efficiency of dendrimer to stabilize spherical copper-doped MgO nanoparticles (ca. 30 %) is higher than in other samples, which was confirmed by UV-Visible, DLS, FTIR, and TEM analysis. The amylase inhibition assay emphasized that the dendrimer nanoparticles stabilization has led to the prolonged enzyme inhibition ability of MgO and copper-doped MgO nanoparticles for up to 24 h.
本工作旨在通过溶胶-凝胶法制备铜掺杂氧化镁纳米粒子，并研究其对未掺杂氧化镁纳米粒子的抗糖尿病α-淀粉酶抑制活性。还评估了 G5 胺端聚酰胺-胺（PAMAM）树枝状大分子控制释放铜掺杂氧化镁纳米粒子以表现出α-淀粉酶抑制活性的能力。通过溶胶-凝胶法合成氧化镁纳米粒子并优化煅烧温度和时间，导致形成具有不同形状（球形、六方和棒状）和尺寸多分散性（10 至 100nm）的纳米粒子，具有尖晶石晶相。氧化镁纳米粒子中铜离子的存在改变了它们的晶粒尺寸，最终改变了它们的尺寸、形态和表面电荷。树枝状大分子稳定球形铜掺杂氧化镁纳米粒子（约 30%）的效率高于其他样品，这通过 UV-可见、DLS、FTIR 和 TEM 分析得到了证实。淀粉酶抑制试验强调，树枝状大分子纳米粒子的稳定导致氧化镁和铜掺杂氧化镁纳米粒子的酶抑制能力延长至 24 小时。
