Wang Jingcai, Yang Lixin, Sun Peng, Guo Chunyan, Jin Yuzi, Jing Xiaoqing
Department of Pediatric, The Affiliated Hospital of Chengde Medical University, Chengde, Hebei, China.
Clin Respir J. 2025 Mar;19(3):e13631. doi: 10.1111/crj.13631. Epub 2023 Jun 29.
Bronchial asthma (BA) is a heterogeneous disease characterized by chronic airway inflammation. This study investigated the serum miR-27a-3p/activating transcription factor 3 (ATF3) expression in children with BA and their correlations with airway inflammation.
Children with BA (N = 120) and healthy children (N = 108) were enrolled. Serum levels of interleukin (IL)-17, IL-6, tumor necrosis factor (TNF)-α, immunoglobulin E (IgE), miR-27a-3p, ATF3, and the number of eosinophils (EOS) were measured using enzyme-linked immunosorbent assay (ELISA), reverse transcription quantitative polymerase chain reaction (RT-qPCR), and an automatic hematology analyzer. The correlations between miR-27a-3p and ATF3 and between miR-27a-3p/ATF3 and inflammation-related factors were analyzed by the Pearson method. The diagnostic values of miR-27a-3p and ATF3 in BA were evaluated using receiver operating characteristic (ROC) curves. The influencing factors of BA were assessed using multivariate logistic regression. Finally, the targeting relation between miR-27a-3p and ATF3 was predicted and analyzed by TargetScan and Starbase databases, and dual-luciferase assay.
There were significant differences in forced expiratory volume in 1 s (FEV1)% predicted and FEV1/forced vital capacity (FVC)%, serum levels of IgE, IL-17, IL-6, and TNF-α, and EOS numbers between healthy children and BA children. Serum miR-27a-3p was negatively correlated with ATF3 and positively correlated with inflammation-related factors in BA children. Serum ATF3 mRNA levels were negatively correlated with inflammatory factors in BA children. miR-27a-3p and ATF3 had good diagnostic values in BA children. FEV% predicted, IL-6, TNF-α, miR-27a-3p, and ATF3 were independent risk factors for BA. miR-27a-3p targeted ATF3.
Serum miR-27a-3p was highly expressed, whereas ATF3 was poorly expressed in BA children, and they were significantly correlated with airway inflammation, had good diagnostic values in BA children, and were independent risk factors for asthma.
支气管哮喘（BA）是一种以慢性气道炎症为特征的异质性疾病。本研究调查了BA患儿血清中miR-27a-3p/激活转录因子3（ATF3）的表达及其与气道炎症的相关性。
纳入BA患儿120例和健康儿童108例。采用酶联免疫吸附测定（ELISA）、逆转录定量聚合酶链反应（RT-qPCR）和自动血液分析仪检测血清白细胞介素（IL）-17、IL-6、肿瘤坏死因子（TNF）-α、免疫球蛋白E（IgE）、miR-27a-3p、ATF3水平及嗜酸性粒细胞（EOS）数量。采用Pearson法分析miR-27a-3p与ATF3之间以及miR-27a-3p/ATF3与炎症相关因子之间的相关性。采用受试者工作特征（ROC）曲线评估miR-27a-3p和ATF3对BA的诊断价值。采用多因素logistic回归评估BA的影响因素。最后，通过TargetScan和Starbase数据库以及双荧光素酶报告基因实验预测并分析miR-27a-3p与ATF3之间的靶向关系。
健康儿童与BA患儿在1秒用力呼气容积（FEV1）占预计值百分比、FEV1/用力肺活量（FVC）百分比、血清IgE、IL-17、IL-6、TNF-α水平及EOS数量方面存在显著差异。BA患儿血清miR-27a-3p与ATF3呈负相关，与炎症相关因子呈正相关。BA患儿血清ATF3 mRNA水平与炎症因子呈负相关。miR-27a-3p和ATF3对BA患儿具有良好的诊断价值。FEV1占预计值百分比、IL-6、TNF-α、miR-27a-3p和ATF3是BA的独立危险因素。miR-27a-3p靶向ATF3。
BA患儿血清miR-27a-3p高表达，而ATF3低表达，它们与气道炎症显著相关，对BA患儿具有良好的诊断价值，是哮喘的独立危险因素。
