Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China; The Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.
Plant Sci. 2023 Oct;335:111678. doi: 10.1016/j.plantsci.2023.111678. Epub 2023 Jun 27.
Rosa chinensis is an important economic and ornamental crop, but powdery mildew greatly reduces its ornamental and economic value. The RcCPR5 gene, encoding a constitutive expressor of pathogenesis-related genes, has two splicing variants in R. chinensis. Compared with RcCPR5-1, RcCPR5-2 has a large C-terminal deletion. During disease development, RcCPR5-2 responded quickly and coordinated with RcCPR5-1 to resist the invasion of the powdery mildew pathogen. In virus-induced gene silencing experiments, down-regulation of RcCPR5 improved the resistance of R. chinensis to powdery mildew. This was confirmed to be broad-spectrum resistance. In the absence of pathogen infection, RcCPR5-1 and RcCPR5-2 formed homodimers and heterodimers to regulate plant growth; but when infected by the powdery mildew pathogen, the RcCPR5-1 and RcCPR5-2 complexes disassociated and released RcSIM/RcSMR to induce effector-triggered immunity, thereby inducing resistance to pathogen infection.
月季是一种重要的经济和观赏作物，但白粉病极大地降低了其观赏和经济价值。RcCPR5 基因，编码一个组成型表达的病程相关基因，在月季中有两种剪接变体。与 RcCPR5-1 相比，RcCPR5-2 具有较大的 C 端缺失。在疾病发展过程中，RcCPR5-2 快速响应，并与 RcCPR5-1 协调，抵抗白粉病病原体的入侵。在病毒诱导的基因沉默实验中，下调 RcCPR5 提高了月季对白粉病的抗性。这被证实是广谱抗性。在没有病原体感染的情况下，RcCPR5-1 和 RcCPR5-2 形成同源二聚体和异源二聚体来调节植物生长；但当感染白粉病病原体时，RcCPR5-1 和 RcCPR5-2 复合物解离并释放 RcSIM/RcSMR 来诱导效应子触发的免疫，从而诱导对病原体感染的抗性。
