National Center of Meat Quality & Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
National Center of Meat Quality & Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
J Microbiol Methods. 2023 Aug;211:106776. doi: 10.1016/j.mimet.2023.106776. Epub 2023 Jun 27.
Efficient and highly controllable antibacterial effects, as well as good biocompatibility, are required for antibacterial materials to overcome multi-drug resistance in bacteria. Herein, mesoporous silica nanomaterials (MSNs) carriers with a particle mean size of 60 nm and pore size of 7.9 nm were prepared, which was followed by loading with D-cysteine (D-Cys) and modified with Polyethyleneimine (PEI) molecules on the outer surface (named as D@MSNs-P). The prepared D@MSNs-P showed a good pH response in the range of 5-7, and the rate of antibacterial agent D-Cys released from nanocarriers was much faster at lower pH (pH 5) than that at higher pH (pH 6-7), which favors the rapid control of the pathogenic bacteria. In a working pH (pH 5), D@MSNs-P exhibited broad-spectrum antibacterial activities against Escherichia coli, Staphylococcus aureus, Salmonella enteritidis, and Listeria monocytogenes with the highest antibacterial efficiency of 99.9%, 99.8%, 98.1%, and 96.2%, respectively, which is much higher than that of pure D-Cys, pure MSNs, D@MSNs, and PEI group. The outstanding antibacterial activity of D@MSNs-P was attributed to the synergistic effect of the unique structure of MSNs and chiral D-Cys molecules. In addition, the prepared D@MSNs-P has no cytotoxicity to HepG2 cells (Human hepatoma cells) at the concentration of 0.4-12.8 mg/mL and even can promote cell proliferation at high concentrations. Our results open a new door for designing the most promising nanomaterials for pH response release and controllable antimicrobial.
高效且高度可控的抗菌效果以及良好的生物相容性是抗菌材料克服细菌多药耐药性所必需的。在此，制备了粒径为 60nm、孔径为 7.9nm 的介孔硅纳米材料（MSNs）载体，然后在其外表面负载 D-半胱氨酸（D-Cys）并进行聚乙烯亚胺（PEI）分子修饰（命名为 D@MSNs-P）。制备的 D@MSNs-P 在 pH 5-7 范围内表现出良好的 pH 响应性，纳米载体中抗菌剂 D-Cys 的释放速率在较低 pH（pH 5）时比在较高 pH（pH 6-7）时快得多，这有利于快速控制病原菌。在工作 pH（pH 5）下，D@MSNs-P 对大肠杆菌、金黄色葡萄球菌、肠炎沙门氏菌和单核细胞增生李斯特菌表现出广谱抗菌活性，抗菌效率最高分别为 99.9%、99.8%、98.1%和 96.2%，明显高于纯 D-Cys、纯 MSNs、D@MSNs 和 PEI 组。D@MSNs-P 的优异抗菌活性归因于 MSNs 的独特结构和手性 D-Cys 分子的协同作用。此外，在浓度为 0.4-12.8mg/mL 时，制备的 D@MSNs-P 对 HepG2 细胞（人肝癌细胞）没有细胞毒性，甚至在高浓度时可以促进细胞增殖。我们的研究结果为设计最有前途的 pH 响应释放和可控抗菌纳米材料开辟了新途径。
