Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Microbiology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
Artif Cells Nanomed Biotechnol. 2020 Nov 16;48(1):1354-1361. doi: 10.1080/21691401.2020.1850466.
The ever-increasing resistance to antimicrobial agents among bacteria associated with nosocomial infections indicate the necessity of new antimicrobial therapy. The nanoparticles are considered as new drug delivery systems to increase the efficiency and decrease the unfavourable effects of the antimicrobial agents.
Herein we report the preparation and characterization of mesoporous silica nanoparticles (MSNs) loaded with meropenem against carbapenem-resistant The antimicrobial effect of meropenem-loaded MSNs was determined against using the minimum inhibitory (MIC) method. The biocompatibility of meropenem-loaded MSNs was studied by the impact on the haemolysis and sedimentation rates of human red blood cells (HRBCs). Cytotoxicity of the meropenem-loaded MSNs was studied by the MTT test (hBM-MSC cell viability).
The meropenem-loaded MSNs have shown antibacterial activity on all isolates at different MIC values lower than MICs of meropenem. Free MSNs did not show any significant antibacterial effect. Meropenem-loaded MSNs have no significant effect on haemolysis and ESR of HRBCs. The viability of hBM-MSC cells treated with serial concentrations of meropenem-loaded MSNs was 92-100%.
Due to the desirable biocompatibility, low cytotoxicity and the improved antibacterial effect, MSNs can be considered as a promising drug delivery system for meropenem as a potential antimicrobial agent.
与医院感染相关的细菌对抗菌药物的耐药性不断增加，表明需要新的抗菌治疗方法。纳米颗粒被认为是新的药物传递系统，可以提高抗菌药物的效率并降低其不良作用。
本文报道了载美罗培南介孔硅纳米颗粒（MSNs）的制备和表征，用于治疗耐碳青霉烯类抗菌药物的 。采用最低抑菌浓度（MIC）法测定载美罗培南 MSNs 的抗菌作用。通过对人红细胞（HRBC）溶血率和沉降率的影响研究载美罗培南 MSNs 的生物相容性。通过 MTT 试验（hBM-MSC 细胞活力）研究载美罗培南 MSNs 的细胞毒性。
载美罗培南 MSNs 对所有分离株均显示出不同 MIC 值的抗菌活性，低于美罗培南的 MIC 值。游离 MSNs 没有显示出任何明显的抗菌作用。载美罗培南 MSNs 对 HRBCs 的溶血率和 ESR 没有显著影响。用载美罗培南 MSNs 的不同浓度处理的 hBM-MSC 细胞的存活率为 92-100%。
由于具有良好的生物相容性、低细胞毒性和改善的抗菌作用，MSNs 可被视为美罗培南的有前途的药物传递系统，作为一种潜在的抗菌药物。
