Rakha B A, Ansari M S, Akhter S, Santiago-Moreno J, Blesbois E
Department of Wildlife Management, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, 46300, Pakistan.
Department of Zoology, University of Lahore-Sargodha Campus, 40100, Pakistan.
Theriogenology. 2018 Oct 1;119:150-155. doi: 10.1016/j.theriogenology.2018.06.015. Epub 2018 Jun 28.
Egg yolk is a good external cryoprotectant of mammalian sperm and some wild bird's sperm, but, at least in domestic breeds of chicken (Gallus gallus domesticus), it may inhibit eventual fertilization of ova when high concentrations are used. We hypothesized that egg yolk can protect the sperm from cryo-induced damages providing adequate fertilization in one phylogenetic wild ancestor of current chicken breeds: the Indian red jungle fowl (Gallus gallus murghi). To test the hypothesis, the present study was designed to evaluate different concentrations of egg yolk in extender in comparison with glycerol. Semen collected from Indian red jungle fowl cocks (n = 8) was cryopreserved using different egg yolk concentrations (10%, 15%, 20% and 25%) or 20% glycerol (control group) following routine protocol of cryopreservation. During cryopreservation, sperm motility (67.5 ± 2.5%), plasma membrane integrity (66.3 ± 2.4%), viability (58.8 ± 1.3%) and acrosomal integrity (60.0.8 ± 2.0%) were recorded highest in an extender with 15% egg yolk compared to other experimental extenders and control at post-dilution, cooling, equilibration and thawing. The no. of fertilized eggs (26.6 ± 0.7, 21.6 ± 1.2), percent fertility (55.9 ± 4.4, 46.5 ± 2.2), no. of hatched chicks (23.6 ± 1.0, 17.2 ± 1.0), percent hatch (49.5 ± 3.2, 37.1 ± 2.5%) and hatchability of the fertile eggs (89.4 ± 2.2, 79.7 ± 3.7) were recorded higher (P < 0.05) with semen cryopreserved with 15% egg yolk compared to control (20% glycerol). It is concluded that 15% egg yolk can be used in cryopreservation protocol of Indian red jungle fowl sperm.
蛋黄是哺乳动物精子和一些野生鸟类精子的良好外部冷冻保护剂，但至少在鸡的家养品种（家鸡）中，当使用高浓度蛋黄时，它可能会抑制卵子的最终受精。我们推测，蛋黄可以保护精子免受冷冻诱导的损伤，从而在现代鸡品种的一个系统发育野生祖先——印度红原鸡中实现充分受精。为了验证这一假设，本研究旨在评估稀释液中不同浓度的蛋黄与甘油相比的效果。按照常规冷冻保存方案，使用不同蛋黄浓度（10%、15%、20%和25%）或20%甘油（对照组）对从印度红原鸡公鸡（n = 8）采集的精液进行冷冻保存。在冷冻保存过程中，与其他实验稀释液和对照组相比，在稀释后、冷却、平衡和解冻时，含有15%蛋黄的稀释液中精子活力（67.5 ± 2.5%）、质膜完整性（66.3 ± 2.4%）、存活率（58.8 ± 1.3%）和顶体完整性（60.0.8 ± 2.0%）记录最高。与对照组（20%甘油）相比，用15%蛋黄冷冻保存的精液受精蛋数量（26.6 ± 0.7，21.6 ± 1.2）、受精率（55.9 ± 4.4，46.5 ± 2.2）、孵化出的雏鸡数量（23.6 ± 1.0，17.2 ± 1.0）、孵化率（49.5 ± 3.2，37.1 ± 2.5%）和受精蛋孵化率（89.4 ± 2.2，79.7 ± 3.7）记录更高（P < 0.05）。得出的结论是，15%的蛋黄可用于印度红原鸡精子的冷冻保存方案。
