Laboratório de Andrologia e Reprodução Animal Assistida - LARAA, Londrina, Paraná, Brazil.
Reprod Domest Anim. 2023 Sep;58(9):1207-1213. doi: 10.1111/rda.14420. Epub 2023 Jul 6.
The use of α2-adrenergic agonists in association with urethral catheterization has been used as a technique for pharmacological semen collection in cats. The mechanism of action of this drug is the stimulation of adrenoreceptors in the vas deferens, which results in ejaculation. While medetomidine is the α2-agonist most commonly used in studies, ejaculation with the use of dexmedetomidine associated with ketamine has been effective, but with variable results. Therefore, further studies regarding the methodology of use are required to obtain better seminal quality. This study aimed to compare two pharmacological semen collection times after the association of dexmedetomidine (30 μg/kg, IM; Dormitor®, Zoetis), ketamine (5 mg/kg, IM; ketamine, Vetnil) and urethral catheterization using a tomcat probe (0.8 mm × 1.00 mm × 11 cm). The collections were divided into two experimental groups: G10 (N = 8; urethral catheterization after 10 min of anaesthesia) and G15 (N = 8; urethral catheterization after 15 min of anaesthesia). The ejaculates were evaluated for ejaculate volume, sperm concentration, morphology and kinetics using the CASA system. To compare the groups, the t-test and the Mann-Whitney U-test were used with a significance level of 5%. It was identified that ejaculate volume (G10: 22.62 ± 2.13 vs. G15: 26.81 ± 1.55; p < .001) and sperm concentration (G10: 48.10 × 10 ± 17.84 vs. G15: 90.18 × 10 ± 19.35; p < .001) was higher in G15 than in G10 and had a lower percentage of minor defects than G10 (G10: 3.12 ± 2.41 vs. G15: 1.00 ± 1.19; p = .043). Regarding the kinetic parameters, the results of G15 were better for total motility-TM (G10: 67.00 ± 10.33 vs. G15: 81.87 ± 7.99; p = .006) and faster cells-RAPID: (G10: 55.00 ± 16.63 vs. G15: 74.25 ± 11.94; p = .019); whereas a higher proportion of cells with slow speed-SLOW were seen in G10 (G10: 31.00 ± 12.07 vs. 17.12 ± 7.53; p = .015). Based on these findings, we suggest that collection via urethral catheterization should be performed 15 min after the application of ketamine-associated dexmedetomidine to obtain a better-quality ejaculate.
在猫的精液采集方面，将α2-肾上腺素能激动剂与尿道导管插入术联合使用已被用作一种药理学精液采集技术。这种药物的作用机制是刺激输精管中的肾上腺素受体，导致射精。虽然在研究中最常使用美托咪定作为 α2-激动剂，但已证明与氯胺酮联合使用右美托咪定（30μg/kg，IM；Dormitor ® ，Zoetis）进行射精是有效的，但结果存在差异。因此，需要进一步研究使用方法，以获得更好的精液质量。本研究旨在比较两种药理学精液采集时间，即在使用右美托咪定（30μg/kg，IM；Dormitor ® ，Zoetis）和氯胺酮（5mg/kg，IM；氯胺酮，Vetnil）后 10 分钟（G10；n=8；尿道导管插入术）和 15 分钟（G15；n=8；尿道导管插入术）进行精液采集。采集的精液通过 CASA 系统评估精液量、精子浓度、形态和动力学。为了比较两组，使用 t 检验和曼-惠特尼 U 检验，显著性水平为 5%。结果表明，G15 组的精液量（G10：22.62±2.13 vs. G15：26.81±2.15；p<.001）和精子浓度（G10：48.10×10 6±17.84 vs. G15：90.18×10 6±19.35；p<.001）均高于 G10 组，并且 G10 组的小缺陷比例低于 G10 组（G10：3.12±2.41 vs. G15：1.00±2.19；p=0.043）。关于动力学参数，G15 组的总活力-TM（G10：67.00±10.33 vs. G15：81.87±7.99；p=0.006）和更快的细胞-RAPID 更好：（G10：55.00±16.63 vs. G15：74.25±11.94；p=0.019）；而 G10 组的慢速细胞-SLOW 比例更高（G10：31.00±12.07 vs. 17.12±7.53；p=0.015）。基于这些发现，我们建议在应用氯胺酮联合右美托咪定后 15 分钟进行尿道导管插入术采集精液，以获得更高质量的精液。
