Rolandelli R H, Koruda M J, Settle R G, Rombeau J L
Surgery. 1986 Aug;100(2):198-204.
The effects of an intracolonic infusion of short-chain fatty acids (SCFA) on the healing of colonic anastomosis in the rat were investigated. Thirty-three Sprague-Dawley rats underwent transection and anastomosis of the descending colon and transection and diversion of the ascending colon. The proximal limb of the ascending colon was exteriorized as an end colostomy, and the distal (defunctionalized) limb was cannulated for continuous infusion. Rats received either no infusion (N = 11) or an infusion of either electrolytes (N = 11) or SCFA (acetate, propionate, and butyrate; N = 11) into the defunctionalized colonic segment. On the sixth postoperative day bursting pressure (BP) and bowel wall tension (BWT) were determined. The occurrence of spontaneous anastomotic dehiscence was significantly less for the SCFA group (0/11) compared with the no infusion group (5/11, p less than 0.01). The anastomotic suture line burst in significantly fewer colons from the SCFA group (1/11) than either the electrolyte infusion (8/11, p less than 0.003) or the no infusion (6/6, p less than 0.001) groups. BP and BWT were significantly higher for the SCFA group (BP: 147 +/- 10 mm Hg; BWT: 59 +/- 1.0 dyne 10(3)/cm) than for either the electrolyte (BP: 99 +/- 30 mm Hg, p less than 0.002; BWT: 45 +/- 19, p less than 0.03) or no infusion (BP: 111 +/- 42, p less than 0.02; BWT 36 +/- 15, p less than 0.007) groups. The results of this study indicate that intracolonic infusion of SCFA resulted in significantly stronger colonic anastomosis in the rat.
研究了结肠内输注短链脂肪酸（SCFA）对大鼠结肠吻合口愈合的影响。33只Sprague-Dawley大鼠接受了降结肠横断吻合术以及升结肠横断和转流术。升结肠的近端作为端式结肠造口术外置，远端（失功能）肢体插管用于持续输注。大鼠分为三组，分别是未输注组（N = 11）、输注电解质组（N = 11）或输注SCFA（乙酸盐、丙酸盐和丁酸盐；N = 11）组，向失功能的结肠段内输注相应物质。术后第6天测定爆破压力（BP）和肠壁张力（BWT）。与未输注组（5/11，p < 0.01）相比，SCFA组（0/11）自发吻合口裂开的发生率显著降低。SCFA组（1/11）吻合口缝线破裂的结肠明显少于电解质输注组（8/11，p < 0.003）或未输注组（6/6，p < 0.001）。SCFA组的BP和BWT（BP：147±10 mmHg；BWT：59±1.0达因10(3)/cm）显著高于电解质组（BP：99±30 mmHg，p < 0.002；BWT：45±19，p < 0.03）或未输注组（BP：111±42，p < 0.02；BWT 36±15，p < 0.007）。本研究结果表明，结肠内输注SCFA可使大鼠结肠吻合口明显增强。
