Zhang Yanan, Zhuang Zhiwei, Mahmood Tahir, Mercier Yves, Jin Yongyan, Huang Xuebing, Li Kaichao, Wang Shuang, Xia Weiguang, Wang Shenglin, Yu Miao, Chen Wei, Zheng Chuntian
Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture, State Key Laboratory of Livestock and Poultry Breeding, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, 510640, Guangzhou, China.
Institute of Life Science and Engineering, Foshan University, 528200, Foshan, China.
Anim Nutr. 2023 May 3;14:101-110. doi: 10.1016/j.aninu.2023.04.006. eCollection 2023 Sep.
This experiment aimed to study the effects of supplemental methionine sources, 2-hydroxy-4 methyl(thio) butanoic acid (HMTBa) and DL-Methionine (DL-Met), on productive performance, egg quality, and redox status of laying ducks. A total of 792 healthy 25-wk-old Longyan laying ducks with similar body weights were randomly allotted to 11 treatment groups. Each treatment group had 6 replicates of 12 ducks. The trial lasted for 16 wk. Ducks were fed a basal deficient diet (Met: 0.24%; Met + Cys: 0.51%) or supplemented with DL-Met or HMTBa at 0.05%, 0.12%, 0.19%, 0.26%, and 0.33% of diet, respectively. Compared with the basal diet, supplementation with either DL-Met or HMTBa increased the average egg weight, egg mass, and decreased feed to egg ratio during the whole trial period ( < 0.05). Albumen weight and its ratio to total egg weight were increased, but yolk and shell ratio, albumen height, Haugh unit and shell breaking strength were decreased ( < 0.05). Dietary DL-Met or HMTBa supplementation increased taurine, methionine, leucine, tryptophan and arginine content, and decreased serine and lysine content in plasma ( < 0.05). The redox status of laying ducks was improved by enhancing the glutathione peroxidase and catalase activities, glutathione content and its ratio relative to glutathione (oxidized) content and decreasing malondialdehyde content and increasing mRNA expression of superoxide dismutase-1, glutathione peroxidase-1, hemeoxygenase-1 and nuclear factor-like 2 in liver and ileum with the supplementation of DL-Met or HMTBa ( < 0.05). Liver health status measured by average area proportion lipid droplet was improved with supplementation of DL-Met or HMTBa ( < 0.05). Villus height and villus height to crypt depth ratio in the ileum and the ileal gene expression of tight junction protein and occludin were increased with DL-Met or HMTBa supplementation ( < 0.05). Taken together, these results suggested that the efficacy of dietary supplementation of HMTBa was similar to DL-Met, and it ranged from 98% to 100% for productive performance and egg albumen ratio in laying ducks (25 to 41 wk).
本试验旨在研究添加蛋氨酸源2-羟基-4-甲基（硫）丁酸（HMTBa）和DL-蛋氨酸（DL-Met）对蛋鸭生产性能、蛋品质及氧化还原状态的影响。选取792只体重相近、健康的25周龄龙岩蛋鸭，随机分为11个处理组。每个处理组有6个重复，每个重复12只鸭。试验期为16周。试验鸭饲喂基础低蛋氨酸日粮（蛋氨酸：0.24%；蛋氨酸+胱氨酸：0.51%），或分别添加占日粮0.05%、0.12%、0.19%、0.26%和0.33%的DL-Met或HMTBa。与基础日粮相比，添加DL-Met或HMTBa均提高了整个试验期的平均蛋重、产蛋量，并降低了料蛋比（P<0.05）。蛋白重及其占总蛋重的比例增加，但蛋黄和蛋壳比例、蛋白高度、哈氏单位和蛋壳强度降低（P<0.05）。日粮中添加DL-Met或HMTBa可提高血浆中牛磺酸、蛋氨酸、亮氨酸、色氨酸和精氨酸含量，降低丝氨酸和赖氨酸含量（P<0.05）。添加DL-Met或HMTBa可提高蛋鸭谷胱甘肽过氧化物酶和过氧化氢酶活性、谷胱甘肽含量及其与谷胱甘肽（氧化型）含量的比值，降低丙二醛含量，并提高肝脏和回肠中超氧化物歧化酶-1、谷胱甘肽过氧化物酶-1、血红素加氧酶-1和核因子样2的mRNA表达，从而改善蛋鸭的氧化还原状态（P<0.05）。添加DL-Met或HMTBa可改善以脂滴平均面积比例衡量的肝脏健康状况（P<0.05）。添加DL-Met或HMTBa可增加回肠绒毛高度和绒毛高度与隐窝深度比值以及紧密连接蛋白和闭合蛋白的回肠基因表达（P<0.05）。综上所述，这些结果表明，日粮添加HMTBa的效果与DL-Met相似，对25至41周龄蛋鸭的生产性能和蛋白比例而言，其效果在98%至100%之间。
