Research Scholar; Department of Genetics, Maharshi Dayanand University, Rohtak, Haryana, 124001, India.
Assistant Director; Directorate of Forensics Services, Himachal Pradesh, Junga-171218, India.
Sovrem Tekhnologii Med. 2023;15(1):63-72. doi: 10.17691/stm2023.15.1.07. Epub 2023 Jan 28.
is to evaluate the suitability of STRs for molecular characterization and forensic applications in unrelated Brahmins of Rajasthan and Haryana states, India.
A total of 203 male DNA samples from various districts of Haryana (n=104) and Rajasthan (n=99) were genotyped using the GlobalFiler PCR Amplification Kit. Allelic frequencies and different forensic parameters like PD, PE, PIC, PM, Ho, He, UHe, and TPI were calculated with different software.
More than 200 alleles were present in both populations, ranging from 6.0 to 35.2 and SE33 was the most polymorphic marker. The combined power of discrimination was 1. To know the relatedness with other Indian Brahmin populations, the UPGMA dendrogram and principal component analysis plot were visualized to show that both populations are close to each other and in nearby Saraswat Brahmins of Himachal Pradesh. This study showed a genetic relationship and forensic examination in the Haryana and Rajasthan Brahmin populations and various ethno-linguistically diverse populations of India.
The results imply that the highly polymorphic 21 autosomal STR loci might be applied for individuals' forensic identification and parentage testing. This study also suggests that the kit having both autosomal and Y-STR markers is appropriate for a better understanding of the genetic and forensic examination in the Brahmin population of Haryana and Rajasthan.
评估 STR 用于分子特征分析和法医应用在印度拉贾斯坦邦和哈里亚纳邦无关婆罗门的适用性。
从哈里亚纳邦（n=104）和拉贾斯坦邦（n=99）的不同地区采集了 203 名男性 DNA 样本，使用 GlobalFiler PCR 扩增试剂盒进行基因分型。使用不同的软件计算等位基因频率和不同的法医参数，如 PD、PE、PIC、PM、Ho、He、UHe 和 TPI。
两个群体都存在 200 多个等位基因，范围从 6.0 到 35.2，SE33 是最多态性的标记。联合判别能力为 1。为了了解与其他印度婆罗门群体的关系，使用 UPGMA 聚类树状图和主成分分析图进行可视化，结果表明两个群体彼此接近，与印度喜马偕尔邦的 Saraswat 婆罗门也接近。本研究显示了哈里亚纳邦和拉贾斯坦邦婆罗门群体的遗传关系和法医检查，以及印度各种种族和语言多样化的群体。
结果表明，高度多态的 21 个常染色体 STR 位点可用于个体的法医鉴定和亲子关系测试。本研究还表明，试剂盒同时具有常染色体和 Y-STR 标记，适用于更好地理解哈里亚纳邦和拉贾斯坦邦婆罗门群体的遗传和法医检查。
