Wang Renjie, Li Qi, Chu Xiaolei, Li Nan, Liang Haiqian, He Feng
Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin, 300072, China.
Department of Neurosurgery, Characteristic Medical Center of Chinese People's Armed Police Force, Tianjin, 300162, China.
Metab Brain Dis. 2023 Oct;38(7):2289-2300. doi: 10.1007/s11011-023-01256-w. Epub 2023 Jun 30.
Evidence suggests that non-coding RNAs have a role in glioblastoma multiforme (GBM), although the regulatory mechanisms controlled by competing endogenous RNAs (ceRNAs) in GBM are still poorly understood and infrequently described. This research extensively analyzed circRNA, lncRNA, miRNA, and mRNA expression changes in GBM patients. RNA-sequencing analyses were conducted to investigate differentially expressed genes (DEGs), lncRNAs (DELs), miRNAs (DEMs), and circRNAs (DECs) in the GBM. In this study, researchers found that GBM patients and healthy controls differed in the presence of 1224 DECs, 1406 DELs, 229 DEMs, and 2740 DEGs. PPI network analysis demonstrated that CEACAM5, CXCL17, FAM83A, TMPRSS4, and GGPRC5A were hub genes and enriched in modules. Then a ceRNA network was constructed with 8 circRNA, 7 lncRNAs, 16 miRNAs, and 17 mRNAs. Overall, the ceRNA interaction axes that were found may prove to be pivotal therapeutic targets for treating GBM.
有证据表明非编码RNA在多形性胶质母细胞瘤（GBM）中发挥作用，尽管由竞争性内源RNA（ceRNA）在GBM中控制的调控机制仍知之甚少且很少被描述。本研究广泛分析了GBM患者中环状RNA（circRNA）、长链非编码RNA（lncRNA）、微小RNA（miRNA）和信使RNA（mRNA）的表达变化。进行RNA测序分析以研究GBM中差异表达基因（DEG）、lncRNA（DEL）、miRNA（DEM）和circRNA（DEC）。在本研究中，研究人员发现GBM患者与健康对照在1224个DEC、1406个DEL、229个DEM和2740个DEG的存在上存在差异。蛋白质-蛋白质相互作用（PPI）网络分析表明癌胚抗原相关细胞黏附分子5（CEACAM5）、趋化因子配体17（CXCL17）、家族性肿瘤相关基因83A（FAM83A）、跨膜丝氨酸蛋白酶4（TMPRSS4）和G蛋白偶联受体C5A（GGPRC5A）是枢纽基因且在模块中富集。然后构建了一个由8个circRNA、7个lncRNA、16个miRNA和17个mRNA组成的ceRNA网络。总体而言，发现了ceRNA相互作用轴可能被证明是治疗GBM的关键治疗靶点。
