Physics Department, Amirkabir University of Technology, P.O. Box 15875-4413, Tehran, Iran.
Physics Department, Amirkabir University of Technology, P.O. Box 15875-4413, Tehran, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 5;302:123055. doi: 10.1016/j.saa.2023.123055. Epub 2023 Jun 22.
Here, the fluorescence properties of some plant-based drug samples are characterized using a coherent excitation source at 405 nm. The laser-induced fluorescence (LIF) spectroscopy is examined to analyze opium and hashish. In order to improve traditional fluorescence methods for better analysis of optically dense materials, we have proposed five characteristic parameters based on solvent densitometry assay as the fingerprints of drugs of interest. The signal emissions are recorded in terms of various drug concentrations, such that the best fitting over experimental data determines the fluorescence extinction (α) and self-quenching (k) coefficients according to the modified Beer-Lambert formalism. The typical α value is determined to be 0.30 and 0.15 mL/(cm∙mg) for opium and hashish, respectively. Similarly, typical k is obtained 0.390 and 1.25 mL/(cm∙mg), respectively. Furthermore, the concentration at max fluorescence intensity (C) is determined for opium and hashish to be 1.8 and 1.3 mg/mL, respectively. Results reveal that opium and hashish benefit their own characteristic fluorescence parameters to discriminate those illicit substances promptly using the present method.
在这里，使用 405nm 的相干激发源对一些植物药样品的荧光性质进行了表征。采用激光诱导荧光（LIF）光谱分析鸦片和大麻。为了改进传统的荧光方法，以便更好地分析光学密度高的材料，我们根据溶剂比重测定法提出了五个基于特征参数作为感兴趣药物的指纹。根据改进的 Beer-Lambert 公式，根据各种药物浓度记录信号发射，从而使实验数据的最佳拟合确定荧光消光（α）和自猝灭（k）系数。对于鸦片和大麻，典型的α值分别确定为 0.30 和 0.15 mL/（cm∙mg）。同样，典型的 k 值分别为 0.390 和 1.25 mL/（cm∙mg）。此外，鸦片和大麻的最大荧光强度（C）浓度分别确定为 1.8 和 1.3mg/mL。结果表明，鸦片和大麻利用自身的特征荧光参数，通过本方法可以快速区分这些非法物质。
