School of Life Sciences, Medical Science and Technology Innovation Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong 250117, PR China.
School of Life Sciences, Medical Science and Technology Innovation Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong 250117, PR China; School of Pharmaceutical Sciences & Institute of Materia Medica, National Key Laboratory of Advanced Drug Delivery System, Medical Science and Technology Innovation Center, Key Laboratory for Biotechnology Drugs of National Health Commission, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong 250117, PR China.
Adv Drug Deliv Rev. 2023 Aug;199:114977. doi: 10.1016/j.addr.2023.114977. Epub 2023 Jun 28.
Organelles perform critical biological functions due to their distinct molecular composition and internal environment. Disorders in organelles or their interacting networks have been linked to the incidence of numerous diseases, and the research of pharmacological actions at the organelle level has sparked pharmacists' interest. Currently, cell imaging has evolved into a critical tool for drug delivery, drug discovery, and pharmacological research. The introduction of advanced imaging techniques in recent years has provided researchers with richer biological information for viewing and studying the ultrastructure of organelles, protein interactions, and gene transcription activities, leading to the design and delivery of precision-targeted drugs. Therefore, this reviews the research on organelles-targeted drugs based upon imaging technologies and development of fluorescent molecules for medicinal purposes. We also give a thorough analysis of a number of subcellular-level elements of drug development, including subcellular research instruments and methods, organelle biological event investigation, subcellular target and drug identification, and design of subcellular delivery systems. This review will make it possible to promote drug research from the individual/cellular level to the subcellular level, as well as give a new focus based on newly found organelle activities.
细胞器由于其独特的分子组成和内部环境而执行关键的生物学功能。细胞器或其相互作用网络的紊乱与许多疾病的发生有关，细胞器水平的药理学作用研究引起了药剂师的兴趣。目前，细胞成像已成为药物输送、药物发现和药理学研究的重要工具。近年来，先进成像技术的引入为研究人员提供了更丰富的生物信息，用于观察和研究细胞器的超微结构、蛋白质相互作用和基因转录活性，从而设计和输送精准靶向药物。因此，本综述基于成像技术和荧光分子在医学上的应用，探讨了靶向细胞器的药物研究进展。我们还对药物开发的一些亚细胞水平的元素进行了全面分析，包括亚细胞研究仪器和方法、细胞器生物事件研究、亚细胞靶点和药物鉴定以及亚细胞递药系统的设计。这一综述将促进从个体/细胞水平到亚细胞水平的药物研究，并基于新发现的细胞器活动提供新的焦点。
