Arroyo-Ramírez Abigail, Lugo-Caballero César, Panti-May J Alonso, Reyes-Novelo Enrique, Rodríguez-Vivas Roger I, Noh-Pech Henry, Suárez-Galaz Alejandro, Osorio-Primo Tana, Puerto Fernando I, Dzul-Rosado Karla, Torres-Castro Marco
Laboratorio de Enfermedades Emergentes y Reemergentes, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, Mexico.
Laboratorio de Zoonosis y otras Enfermedades Transmitidas por Vector, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, Mexico.
Zoonoses Public Health. 2023 Nov;70(7):594-603. doi: 10.1111/zph.13068. Epub 2023 Jun 30.
Rickettsia parkeri belongs to the spotted fever group (SFG) of the Rickettsia genus. This bacterium causes mild rickettsiosis in humans and is mainly transmitted by Amblyomma ticks. Its medical importance is emerging in the Americas, including Mexico. Synanthropic rodents and domiciled dogs participate as accidental hosts in epidemiological cycles of Rickettsia of the SFG. The aim is to report the presence of R. parkeri in synanthropic rodents and domiciled dogs from a rural community of Yucatán, Mexico. Rodents were captured, and plasma samples were taken from dogs in 48 households from Ucú, Yucatán, Mexico. A spleen sample (rodents) and plasma (dogs) were used in the propagation of Rickettsia on Vero cells. These infected cells were used in the extraction of genomic DNA. Rickettsia DNA was identified using a semi-nested PCR (snPCR); some products were sent for sequencing. The recovered sequences were analysed with bioinformatics programs, and a phylogenetic tree was built to determine the Rickettsia species. One hundred animals were sampled: 36 synanthropic rodents and 64 dogs. The snPCR evidenced the presence of Rickettsia DNA in 10 rodents (10/36, 27.8%) and 18 dogs (18/64, 28.1%), which represents a global frequency of 28% (28/100) in this study. The bioinformatics analysis yielded homology to R. parkeri and was demonstrated in the phylogenetic tree. The first evidence of the presence of R. parkeri in synanthropic rodents (Mus musculus) from Mexico is presented; likewise, the participation of domestic dogs in the transmission cycle of this bacterium with potential importance in public health is confirmed.
帕克立克次体属于立克次体属的斑点热群（SFG）。这种细菌可引起人类轻度立克次体病，主要通过美洲钝眼蜱传播。在包括墨西哥在内的美洲地区，其医学重要性日益凸显。共生啮齿动物和家养犬作为偶然宿主参与了SFG立克次体的流行病学循环。目的是报告在墨西哥尤卡坦州一个农村社区的共生啮齿动物和家养犬中帕克立克次体的存在情况。在墨西哥尤卡坦州乌库的48户家庭中捕获了啮齿动物，并采集了犬的血浆样本。用脾脏样本（啮齿动物）和血浆（犬）在Vero细胞上进行立克次体的培养。这些感染的细胞用于基因组DNA的提取。使用半巢式PCR（snPCR）鉴定立克次体DNA；一些产物送去测序。用生物信息学程序分析回收的序列，并构建系统发育树以确定立克次体的种类。共对100只动物进行了采样：36只共生啮齿动物和64只犬。snPCR证明在10只啮齿动物（10/36，27.8%）和18只犬（18/64，28.1%）中存在立克次体DNA，在本研究中这代表了28%（28/100）的总体检出率。生物信息学分析显示与帕克立克次体具有同源性，并在系统发育树中得到证实。本文首次提供了墨西哥共生啮齿动物（小家鼠）中存在帕克立克次体的证据；同样，也证实了家养犬在这种细菌传播循环中的作用，这对公共卫生具有潜在重要性。
