Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD 4072, Australia.
School of Chemical Engineering, The University of Adelaide, Adelaide, SA 5005, Australia.
Adv Colloid Interface Sci. 2023 Aug;318:102957. doi: 10.1016/j.cis.2023.102957. Epub 2023 Jun 26.
Enzymes are widely used in industry due to their high efficiency and selectivity. However, their low stability during certain industrial processes can result in a significant loss of catalytic activity. Encapsulation is a promising technique that can stabilize enzymes by protecting them from environmental stresses such as extreme temperature and pH, mechanical force, organic solvents, and proteases. Alginate and alginate-based materials have emerged as effective carriers for enzyme encapsulation due to their biocompatibility, biodegradability, and ability to form gel beads through ionic gelation. This review presents various alginate-based encapsulation systems for enzyme stabilization and explores their applications in different industries. We discuss the preparation methods of alginate encapsulated enzymes and analyze the release mechanisms of enzymes from alginate materials. Additionally, we summarize the characterization techniques used for enzyme-alginate composites. This review provides insights into the use of alginate encapsulation as a means of stabilizing enzymes and highlights the potential benefits for various industrial applications.
酶由于其高效性和选择性而被广泛应用于工业中。然而,在某些工业过程中,它们的低稳定性会导致催化活性的显著损失。包封是一种很有前途的技术,可以通过保护酶免受环境压力(如极端温度和 pH 值、机械力、有机溶剂和蛋白酶)的影响来稳定酶。由于具有生物相容性、可生物降解性以及通过离子凝胶形成凝胶珠的能力,海藻酸盐和基于海藻酸盐的材料已成为酶包封的有效载体。本综述介绍了各种基于海藻酸盐的酶稳定化包封系统,并探讨了它们在不同行业中的应用。我们讨论了海藻酸盐包封酶的制备方法,并分析了酶从海藻酸盐材料中的释放机制。此外,我们总结了用于酶-海藻酸盐复合材料的表征技术。本综述提供了对海藻酸盐包封作为稳定酶的手段的深入了解,并强调了其在各种工业应用中的潜在好处。
Adv Colloid Interface Sci. 2023-8
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