Weng Yilun, Chen Rui, Hui Yue, Chen Dong, Zhao Chun-Xia
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD 4072, Australia.
School of Chemical Engineering, The University of Adelaide, Adelaide, SA 5005, Australia.
Chem Bio Eng. 2024 Feb 8;1(2):99-112. doi: 10.1021/cbe.3c00091. eCollection 2024 Mar 28.
Enzymes, as highly efficient biocatalysts, excel in catalyzing diverse reactions with exceptional activity and selective properties under mild conditions. Nonetheless, their broad applications are hindered by their inherent fragility, including low thermal stability, limited pH tolerance, and sensitivity to organic solvents and denaturants. Encapsulating enzymes within metal-organic frameworks (MOFs) can protect them from denaturation in these harsh environments. However, this often leads to a compromised enzyme activity. In recent years, extensive research efforts have been dedicated to enhancing enzymatic activity within MOFs, leading to the development of new enzyme-MOF composites that not only preserve their catalytic potential but also outperform their free counterparts. This Review provides a comprehensive review on recent developments in enzyme-MOF composites with a specific emphasis on their enhanced enzymatic activity compared to free enzymes.
酶作为高效的生物催化剂，在温和条件下能够以卓越的活性和选择性催化各种反应。然而，其广泛应用受到自身固有脆弱性的阻碍，包括热稳定性低、pH耐受性有限以及对有机溶剂和变性剂敏感。将酶封装在金属有机框架（MOF）中可以保护它们在这些恶劣环境中不发生变性。然而，这通常会导致酶活性受损。近年来，人们进行了广泛的研究，致力于提高MOF内的酶活性，从而开发出新型的酶-MOF复合材料，这些复合材料不仅保留了催化潜力，而且比游离酶表现更优。本综述全面回顾了酶-MOF复合材料的最新进展，特别强调了与游离酶相比其增强的酶活性。
