Malatová Z, Longauer F, Martiniak J, Marsala J
Act Nerv Super (Praha). 1986 Jun;28(2):87-95.
Activities of choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) in the ventral spinal cord, ventral spinal roots and in the central and peripheral stumps of the sciatic nerve transected under conditions of partial ischemia (produced by aortic ligation just below the renal arteries) were compared to those obtained under intact blood supply in time intervals 5, 10, or 15 days after surgery. The significant increase of ChAT activity in the central part of the sciatic nerve following 15 days of partial ischemia correlated with less significant elevation of ChAT in the ventral spinal cord. The changes of AChE activity were not significant during partial ischemia. ChAT in the peripheral stump of the sciatic nerve following 5 days of partial ischemia was preserved by 40% and AChE by 20% more than under normal blood supply. On the contrary, in the next 5 days interval losses of enzymes activity in the degenerating nerve were greater. ChAT was almost totally inactivated whereas 50% of AChE activity was preserved until the end of period examined.
在部分缺血（由肾动脉下方的主动脉结扎产生）条件下切断坐骨神经后，比较了术后5、10或15天腹侧脊髓、腹侧脊神经根以及坐骨神经中枢端和外周端中胆碱乙酰转移酶（ChAT）和乙酰胆碱酯酶（AChE）的活性，与在完整血液供应条件下获得的活性进行比较。部分缺血15天后，坐骨神经中枢部分ChAT活性显著增加，而腹侧脊髓中ChAT升高不太显著。部分缺血期间AChE活性变化不显著。部分缺血5天后，坐骨神经外周端的ChAT比正常血液供应时多保留40%，AChE多保留20%。相反，在接下来的5天间隔内，退变神经中酶活性的损失更大。ChAT几乎完全失活，而直到检查期结束时仍保留50%的AChE活性。
