Malatová Z, Longauer F, Kundrát I, Martiniak J
Physiol Bohemoslov. 1987;36(2):153-60.
Choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activity measured in the ventral and dorsal part of the dog spinal cord (L6-S2) and in the stumps of the sciatic nerve 5, 10, 15 and 21 days after its transection were compared with the corresponding activities in the intact contralateral nerve and in sham-operated animals. AChE was also examined histochemically. Changes in the enzyme activities in the central nerve stump were correlated with activity changes in the spinal cord. In the central nerve stump, a marked (25%) increase in AChE activity was found on the fifth day after transection, but by the 21st day it fell below control value levels; up to the 15th day it showed good correlation with AChE activity in the ventral spinal cord. Histochemically, pronounced reduction of enzymatic activity was found in the ipsilateral part of the spinal cord. On the 15th day, ChAT activity in the ventral spinal cord was also significantly decreased and the accumulation of the enzyme in the central nerve stump was negligible. On the contrary, at the last 21-day interval examined, a significant increase in ChAT activity and a nonsignificant increase in AChE activity was found in the spinal cord, but their activities in the central nerve stump were decreased. In the degenerated peripheral nerve stump ChAT activity dropped by an average of 99% and AChE activity by 48% during the first 15 days after transection but, on the 21st day, AChE activity was 22% higher than at the preceding interval.
在犬脊髓（L6 - S2）腹侧和背侧以及坐骨神经横断后5、10、15和21天的神经残端中测量胆碱乙酰转移酶（ChAT）和乙酰胆碱酯酶（AChE）活性，并将其与完整对侧神经和假手术动物中的相应活性进行比较。还对AChE进行了组织化学检查。中枢神经残端中酶活性的变化与脊髓中的活性变化相关。在中枢神经残端，横断后第5天发现AChE活性显著增加（25%），但到第21天其降至对照值水平以下；直至第15天，它与脊髓腹侧的AChE活性显示出良好的相关性。组织化学检查发现，脊髓同侧部分的酶活性明显降低。在第15天，脊髓腹侧的ChAT活性也显著降低，且该酶在中枢神经残端中的积累可忽略不计。相反，在最后检查的21天间隔时，脊髓中ChAT活性显著增加，AChE活性有不显著增加，但其在中枢神经残端中的活性降低。在变性的外周神经残端中，横断后的前15天内ChAT活性平均下降99%，AChE活性下降48%，但在第21天，AChE活性比前一间隔高22%。
