Jinan University, College of Pharmacy, Guangzhou, 510632, China.
National Sun yat-sen University, Public Health Hospital (Shenzhen), Shenzhen, 518107, China.
Virus Genes. 2023 Oct;59(5):716-722. doi: 10.1007/s11262-023-01996-y. Epub 2023 Jul 3.
This study aims to screen and identify specific cluster miRNAs of H7N9 virus-infected N2a cells and explore the possible pathogenesis of these miRNAs. The N2a cells are infected with H7N9 and H1N1 influenza viruses, and the cells are collected at 12, 24 and 48 h to extract total RNA. To sequence miRNAs and identify different virus-specific miRNAs, high-throughput sequencing technology is used. Fifteen H7N9 virus-specific cluster miRNAs are screened, and eight of them are included in the miRBase database. These cluster-specific miRNAs regulate many signaling pathways, such as the PI3K-Akt signaling pathway, the RAS signaling pathway, the cAMP signaling pathway, actin cytoskeleton regulation and cancer-related genes. The study provides a scientific basis for the pathogenesis of H7N9 avian influenza, which is regulated by miRNAs.
本研究旨在筛选和鉴定 H7N9 病毒感染的 N2a 细胞中特定的簇 miRNA，并探讨这些 miRNA 的可能发病机制。用 H7N9 和 H1N1 流感病毒感染 N2a 细胞，分别在 12、24 和 48 h 收集细胞提取总 RNA。为了对 miRNA 进行测序并鉴定不同病毒特异性的 miRNA，使用高通量测序技术。筛选出 15 个 H7N9 病毒特异性簇 miRNA，其中 8 个包含在 miRBase 数据库中。这些簇特异性 miRNA 调节许多信号通路，如 PI3K-Akt 信号通路、RAS 信号通路、cAMP 信号通路、肌动蛋白细胞骨架调节和癌症相关基因。该研究为 miRNA 调控的 H7N9 禽流感发病机制提供了科学依据。
