糖尿病：采用布鲁梅（茜草科）水提取物对Wistar大鼠进行预防和治疗的疗法。
Diabetes mellitus: Preventive and curative therapies with aqueous extract of Blume (Rubiaceae) in Wistar rats.
作者信息
Maidadi Barthelemy, Ntchapda Fidèle, Miaffo David, Mahamad Abba Talba
机构信息
Laboratory of Animal Physiology and Pharmacognosy, Department of Biological Sciences, Faculty of Sciences, University of Maroua, P.O. Box 814, Maroua, Cameroon.
Laboratory of Medicinal Plants, Health and Galenic Formulation, Department of Biological Sciences, Faculty of Sciences, University of Ngaoundere, P.O. Box 454, Ngaoundere, Cameroon.
出版信息
J Tradit Complement Med. 2023 Mar 1;13(4):358-367. doi: 10.1016/j.jtcme.2023.03.001. eCollection 2023 Jul.
BACKGROUND AND AIM
African traditional healers use Blume to treat diseases such as diabetes mellitus, malaria, dysentery, constipation, and hemorrhoids. This study aimed to assess the hypoglycemic, lipid-lowering, and antioxidant properties of extract (AERS) in type 1 diabetic (T1D) and insulin-resistant (T2D) rats.
EXPERIMENTAL PROCEDURE
The induction of T1D was made by intraperitoneal administration of streptozotocin (55 mg/kg b.w). As for T2D, it was induced for 10 days by daily subcutaneous administration of dexamethasone (1 mg/kg b.w). Diabetic animals were divided and treated with AERS (50, 100, and 200 mg/kg b.w) for 28 and 10 days for T1D and T2D, respectively. Glycaemia, food and water consumption, relative body weight, insulinemia, lipid profile, and oxidative stress parameters were evaluated. Histological sections were made on the pancreas of T1D rats.
RESULTS AND CONCLUSION
AERS (100 and/or 200 mg/kg) prevented (p < 0.05 to p < 0.001) weight loss, polyphagia, and polysipsia in diabetic rats. AERS significantly lowered (p < 0.05 to p < 0.001) insulinemia, hyperglycemia, triglycerides (TG), low-density lipoprotein cholesterol (LDL-c) total cholesterol (TC),and malondialdehyde (MDA). In contrast, a significant increase (p < 0.05 to p < 0.001) in high-density lipoprotein cholesterol (HDL-c) levels, reduced glutathione levels, and superoxide dismutase (SOD) and catalase (CAT) activities were observed with all doses of AERS. Histopathological analysis showed an increase in the number and size of islets of Langerhans in the pancreas of T1D rats receiving AERS. AERS has an important antidiabetic, antidyslipidemic, and antioxidant potential.
背景与目的
非洲传统治疗师使用布鲁梅属植物治疗糖尿病、疟疾、痢疾、便秘和痔疮等疾病。本研究旨在评估布鲁梅属植物提取物（AERS）对1型糖尿病（T1D）和胰岛素抵抗型（T2D）大鼠的降血糖、降血脂和抗氧化特性。
实验步骤
通过腹腔注射链脲佐菌素（55毫克/千克体重）诱导T1D。至于T2D，通过每日皮下注射地塞米松（1毫克/千克体重）诱导10天。将糖尿病动物分组，分别用AERS（50、100和200毫克/千克体重）治疗28天（T1D）和10天（T2D）。评估血糖、食物和水消耗、相对体重、胰岛素血症、血脂谱和氧化应激参数。对T1D大鼠的胰腺进行组织学切片。
结果与结论
AERS（100和/或200毫克/千克）可预防（p<0.05至p<0.001）糖尿病大鼠体重减轻、多食和多饮。AERS显著降低（p<0.05至p<0.001）胰岛素血症、高血糖、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-c）、总胆固醇（TC）和丙二醛（MDA）。相反，所有剂量的AERS均使高密度脂蛋白胆固醇（HDL-c）水平、还原型谷胱甘肽水平以及超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性显著增加（p<0.05至p<0.001）。组织病理学分析显示，接受AERS的T1D大鼠胰腺中胰岛的数量和大小增加。AERS具有重要的抗糖尿病、抗血脂异常和抗氧化潜力。
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