INTRODUCTION

A safe bio-preservative agent, lactic acid bacteria (LAB) can inhibit the growth of pathogenic bacteria and spoilage organisms. Its cell-free supernatant (LAB-CFS), which is rich in bioactive compounds, is what makes LAB antibacterial work.

METHODS

This study focused on the changes in biofilm activity and related metabolic pathways of treated with lactic acid bacteria planktonic CFS (LAB-pk-CFS) and biofilm state (LAB-bf-CFS).

RESULTS

The findings demonstrated that the LAB-CFS treatment considerably slowed () growth and prevented it from forming biofilms. Additionally, it inhibits the physiological traits of the biofilm, including hydrophobicity, motility, eDNA, and PIA associated to the biofilm. The metabolites of biofilm treated with LAB-CFS were greater in the LAB-bf-CFS than they were in the LAB-pk-CFS, according to metabolomics studies. Important metabolic pathways such amino acids and carbohydrates metabolism were among the most noticeably altered metabolic pathways.

DISCUSSION

These findings show that LAB-CFS has a strong potential to combat infections.