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[物质名称]的无细胞上清液对[生物名称]生物膜及其代谢产物的影响。

Impact of cell-free supernatant of on biofilm and its metabolites.

作者信息

Mao Yanni, Wang Yuxia, Luo Xiaofeng, Chen Xiaohui, Wang Guiqin

机构信息

Veterinary Pharmacology Lab, School of Animal Science and Technology, Ningxia University, Yinchuan, China.

出版信息

Front Vet Sci. 2023 Jun 15;10:1184989. doi: 10.3389/fvets.2023.1184989. eCollection 2023.

DOI:10.3389/fvets.2023.1184989
PMID:37397004
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10310794/
Abstract

INTRODUCTION

A safe bio-preservative agent, lactic acid bacteria (LAB) can inhibit the growth of pathogenic bacteria and spoilage organisms. Its cell-free supernatant (LAB-CFS), which is rich in bioactive compounds, is what makes LAB antibacterial work.

METHODS

This study focused on the changes in biofilm activity and related metabolic pathways of treated with lactic acid bacteria planktonic CFS (LAB-pk-CFS) and biofilm state (LAB-bf-CFS).

RESULTS

The findings demonstrated that the LAB-CFS treatment considerably slowed () growth and prevented it from forming biofilms. Additionally, it inhibits the physiological traits of the biofilm, including hydrophobicity, motility, eDNA, and PIA associated to the biofilm. The metabolites of biofilm treated with LAB-CFS were greater in the LAB-bf-CFS than they were in the LAB-pk-CFS, according to metabolomics studies. Important metabolic pathways such amino acids and carbohydrates metabolism were among the most noticeably altered metabolic pathways.

DISCUSSION

These findings show that LAB-CFS has a strong potential to combat infections.

摘要

引言

乳酸菌（LAB）作为一种安全的生物防腐剂，能够抑制病原菌和腐败微生物的生长。其富含生物活性化合物的无细胞上清液（LAB-CFS）是LAB发挥抗菌作用的关键所在。

方法

本研究聚焦于经乳酸菌浮游细胞无细胞上清液（LAB-pk-CFS）和生物膜状态（LAB-bf-CFS）处理后的生物膜活性及相关代谢途径的变化。

结果

研究结果表明，LAB-CFS处理显著减缓了（）的生长，并阻止其形成生物膜。此外，它还抑制了生物膜的生理特性，包括与生物膜相关的疏水性、运动性、胞外DNA和PIA。代谢组学研究显示，经LAB-CFS处理的生物膜代谢产物在LAB-bf-CFS中比在LAB-pk-CFS中更多。重要的代谢途径，如氨基酸和碳水化合物代谢，是变化最为显著的代谢途径之一。

讨论

这些发现表明，LAB-CFS在对抗（）感染方面具有强大的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8304/10310794/179dc2dc64f3/fvets-10-1184989-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8304/10310794/07cab552958a/fvets-10-1184989-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8304/10310794/8ac021e6c509/fvets-10-1184989-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8304/10310794/0ba92b52d5ce/fvets-10-1184989-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8304/10310794/f9bbc2d40cbf/fvets-10-1184989-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8304/10310794/81e3d434dff6/fvets-10-1184989-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8304/10310794/179dc2dc64f3/fvets-10-1184989-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8304/10310794/07cab552958a/fvets-10-1184989-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8304/10310794/1f741c839ace/fvets-10-1184989-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8304/10310794/477e2d21353a/fvets-10-1184989-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8304/10310794/8ac021e6c509/fvets-10-1184989-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8304/10310794/0ba92b52d5ce/fvets-10-1184989-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8304/10310794/f9bbc2d40cbf/fvets-10-1184989-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8304/10310794/179dc2dc64f3/fvets-10-1184989-g010.jpg

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