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整合代谢组学和肽组学以描绘新型L3发酵乳中的特征性代谢产物

Integrated metabolomics and peptidomics to delineate characteristic metabolites in milk fermented with novel L3.

作者信息

Wang Teng, Wei Guangqiang, Chen Faqiang, Ma Qingwen, Huang Aixiang

机构信息

College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, Yunnan, China.

Yunnan Normal University, Kunming 650092, Yunnan, China.

出版信息

Food Chem X. 2023 Jun 1;18:100732. doi: 10.1016/j.fochx.2023.100732. eCollection 2023 Jun 30.

Abstract

A novel wild-type () L3 with good fermentation characteristics and protein degradation capacity was isolated from raw milk samples. In this study, the metabolites in milk fermented with L3 were investigated by metabolomic and peptidomics analyses. The metabolomics results revealed that the metabolites in milk fermented with L3 were Thr-Pro, Val-Lys, l-creatine, pyridoxine, and muramic acid, which improved the taste and nutritional qualities of the milk. Moreover, the water-soluble peptides derived from L3 fermented milk exhibited high antioxidant properties and angiotensin I-converting enzyme inhibitory (ACEI) activities. Additionally, 152 peptides were found using liquid chromatography-mass spectrometry (LC-MS/MS). Furthermore, endogenous enzymes secreted by L3 cleaved - and -casein to release six ACEI peptides (ACEIPs), nineteen antioxidant peptides (AOPs), and five antimicrobial peptides (AMPS). Overall, these findings could be valuable in improving the quality of fermented milk.

摘要

从生鲜乳样品中分离出一株具有良好发酵特性和蛋白质降解能力的新型野生型()L3。在本研究中,通过代谢组学和肽组学分析对用L3发酵的牛奶中的代谢产物进行了研究。代谢组学结果显示,用L3发酵的牛奶中的代谢产物有苏氨酸-脯氨酸、缬氨酸-赖氨酸、左旋肌酸、吡哆醇和胞壁酸,这些物质改善了牛奶的口感和营养品质。此外,L3发酵乳产生的水溶性肽具有较高的抗氧化性能和血管紧张素I转换酶抑制(ACEI)活性。另外,使用液相色谱-质谱联用(LC-MS/MS)发现了152种肽。此外,L3分泌的内源性酶切割了β-和κ-酪蛋白,释放出6种ACEI肽(ACEIPs)、19种抗氧化肽(AOPs)和5种抗菌肽(AMPs)。总体而言,这些发现对于提高发酵乳的品质可能具有重要价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6297/10314206/e08a53c10622/ga1.jpg

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