Williams Michael, Green George B H, Palmer Joseph W, Fay Christian X, Chehade Sophie B, Lawrence Addison L, Barry Robert J, Powell Mickie L, Harris Melissa L, Watts Stephen A
University of Alabama at Birmingham.
Texas A&M University.
Res Sq. 2023 Jun 14:rs.3.rs-3044822. doi: 10.21203/rs.3.rs-3044822/v1.
Effective use of as a preclinical model requires standardization of macronutrient sources to achieve scientific reproducibility across studies and labs. Our objective was to evaluate single cell protein (SCP) for production of open-source standardized diets with defined heath characteristics for the zebrafish research community. We completed a 16-week feeding trial using juvenile 31 days post-fertilization (dpf) (10 tanks per diet, 14 per tank) with formulated diets containing either a typical fish protein ingredient or a novel bacterial SCP source. At the end of the feeding trial, growth metrics, body composition, reproductive success, and bulk transcriptomics of the liver (RNAseq on female only with confirmatory rtPCR) were performed for each diet treatment.
fed the SCP containing diet had body weight gains equivalent to the fed fish protein, and females had significantly lower total carcass lipid, indicating reduced adiposity. Reproductive success was similar between treatments. Genes differentially expressed in female provided the bacterial SCP compared to females given fish protein were overrepresented in the gene ontologies of metabolism, biosynthesis of cholesterol precursors and products, and protein unfolding responses.
These data support the development of an open-source diet utilizing an ingredient that correlates with improved health profiles and reduced variability in notable outcomes.
将[未提及的某种生物]有效用作临床前模型需要对常量营养素来源进行标准化，以实现跨研究和实验室的科学可重复性。我们的目标是评估单细胞蛋白（SCP），以为斑马鱼研究群体生产具有明确健康特征的开源标准化饲料。我们使用受精后31天（dpf）的幼鱼进行了为期16周的喂养试验（每种饲料10个鱼缸，每个鱼缸14条鱼），饲料配方中含有典型的鱼类蛋白成分或新型细菌SCP来源。在喂养试验结束时，对每种饲料处理进行了生长指标、身体组成、繁殖成功率以及肝脏的整体转录组学分析（仅对雌性进行RNA测序，并通过逆转录聚合酶链反应进行验证）。
喂食含SCP饲料的[未提及的某种生物]体重增加与喂食鱼类蛋白的[未提及的某种生物]相当，并且雌性的胴体总脂质显著降低，表明肥胖程度降低。各处理之间的繁殖成功率相似。与喂食鱼类蛋白的雌性相比，喂食细菌SCP的雌性[未提及的某种生物]中差异表达的基因在代谢、胆固醇前体和产物的生物合成以及蛋白质解折叠反应的基因本体中过度富集。
这些数据支持开发一种开源饲料，该饲料使用的成分与改善健康状况和减少显著结果的变异性相关。
