Department of Pathology, Stanford Health Care, Stanford, CA 94305, United States.
Stanford Hospital and Clinics, Stanford, CA 94305, United States.
Clin Chim Acta. 2023 Aug 1;548:117464. doi: 10.1016/j.cca.2023.117464. Epub 2023 Jul 1.
Peripheral venous blood (PVB) gas analysis has become an alternative to arterial blood gas (BG) analysis in assessing acid-base balance. This study aimed to compare the effects of blood collection devices and modes of transportation on peripheral venous BG parameters.
PVB-paired specimens were collected from 40 healthy volunteers into blood gas syringes (BGS) and blood collection tubes (BCT), transported by either a pneumatic tube system (PTS) or human courier (HC) to the clinical laboratory, and compared using a two-way ANOVA or Wilcoxon signed-rank test. To determine clinical significance, the PTS and HC-transported BGS and BCT biases were compared to the total allowable error (TEA).
PVB partial pressure of oxygen (pO), fractional oxyhemoglobin (FOHb), fractional deoxyhemoglobin (FHHb), and oxygen saturation (sO) showed statistically significant differences between BGS and BCT (p < 0.0001). Compared to HC-transported BGS and BCT, statistically significant increases in pO, FOHb, sO, oxygen content (only in BCT) (all p < 0.0001), and base excess extracellular (only in BCT; p < 0.0014) concentrations and a statistically significant decrease in FHHb concentration (p < 0.0001) were found in BGS and BCT delivered by PTS. The biases between PTS- and HC-transported BGS and BCT exceeded the TEA for many BG parameters.
Collecting PVB in BCT is unsuitable for pO, sO, FOHb, FHHb, and oxygen content determinations.
外周静脉血（PVB）气体分析已成为评估酸碱平衡的动脉血气（BG）分析的替代方法。本研究旨在比较不同采血装置和运输方式对周围静脉 BG 参数的影响。
将 40 名健康志愿者的外周静脉血标本分别采集到血气注射器（BGS）和采血管（BCT）中，通过气动输送系统（PTS）或人工传递（HC）两种方式运输至临床实验室，采用双向方差分析或 Wilcoxon 符号秩检验进行比较。为确定临床意义，将 PTS 和 HC 运输的 BGS 和 BCT 偏差与总允许误差（TEA）进行比较。
BGS 和 BCT 之间的 PVB 部分氧分压（pO2）、氧合血红蛋白分数（FOHb）、脱氧血红蛋白分数（FHHb）和氧饱和度（sO2）有统计学差异（p<0.0001）。与 HC 运输的 BGS 和 BCT 相比，PTS 运输的 BGS 和 BCT 的 pO2、FOHb、sO2、氧含量（仅在 BCT 中）（均 p<0.0001）、碱剩余细胞外液（仅在 BCT；p<0.0014）浓度显著增加，而 FHHb 浓度显著降低（p<0.0001）（均 p<0.0001）。PTS 与 HC 运输的 BGS 和 BCT 之间的偏差超过了许多 BG 参数的 TEA。
在 BCT 中采集 PVB 不适合用于 pO2、sO2、FOHb、FHHb 和氧含量的测定。
