Liu Ying-Hao, Gong Qian, Wang Yi-Kai, Shuang Wei-Bing
First Clinical Medical College, Shanxi Medical University, Taiyuan, China.
Department of Urology, The First Hospital of Shanxi Medical University, Taiyuan, China.
Int Neurourol J. 2023 Jun;27(2):88-98. doi: 10.5213/inj.2346068.034. Epub 2023 Jun 30.
The main treatment options of neurogenic bladder remains catheterization and long-term oral medications. Metabolic interventions have shown good therapeutic results in many diseases. To date, no studies have characterized the metabolites of the detrusor muscle during neurogenic bladder. Using metabolomics, new muscle metabolomic signatures were identified to reveal the temporal metabolic profile of muscle during disease progression.
We used 42 Sprague-Dawley rats (200±20 g, males) for T10 segmental spinal cord injury modeling and collected detrusor tissue and performed nontargeted metabolomics after sham surgery, 30-minute, 6-hour, 12-hour, 24-hour, 5-day, and 2-week postmodelling, to identify the dysregulated metabolic pathways and key metabolites.
By comparing mzCloud, mzVault, MassList, we identified a total of 1,271 metabolites and enriched a total of 12 metabolism-related pathways with significant differences (P<0.05) based on Kyoto Encyclopedia of Genes and Genomes analysis. Metabolites in several differential metabolic pathways such as ascorbate and aldarate metabolism, Steroid hormone biosynthesis, and carbon metabolism are altered in a regular manner before and after ridge shock.
Our study is the first time-based metabolomic study of rat forced urinary muscle after traumatic spinal cord injury, and we identified multiple differential metabolic pathways during injury that may improve long-term management strategies for neurogenic bladder and reduce costs in long-term treatment.
神经源性膀胱的主要治疗选择仍然是导尿和长期口服药物。代谢干预在许多疾病中已显示出良好的治疗效果。迄今为止，尚无研究对神经源性膀胱逼尿肌的代谢物进行表征。利用代谢组学，识别出新的肌肉代谢组学特征，以揭示疾病进展过程中肌肉的时间代谢谱。
我们使用42只Sprague-Dawley大鼠（200±20 g，雄性）进行T10节段脊髓损伤建模，并在假手术、建模后30分钟、6小时、12小时、24小时、5天和2周收集逼尿肌组织并进行非靶向代谢组学分析，以识别失调的代谢途径和关键代谢物。
通过比较mzCloud、mzVault、MassList，我们共鉴定出1271种代谢物，并基于京都基因与基因组百科全书分析富集了12条具有显著差异（P<0.05）的与代谢相关的途径。在脊休克前后，几种差异代谢途径中的代谢物，如抗坏血酸和醛糖代谢、类固醇激素生物合成和碳代谢，均有规律地发生改变。
我们的研究是首次对创伤性脊髓损伤后大鼠逼尿肌进行基于时间的代谢组学研究，并且我们识别出了损伤期间的多种差异代谢途径，这可能会改善神经源性膀胱的长期管理策略并降低长期治疗成本。
