Chen Chao, Yang Qiang, Ma Xinlong
Department of Spine Surgery, Tianjin Hospital, Tianjin, 300211 China.
3 Biotech. 2020 Feb;10(2):50. doi: 10.1007/s13205-019-2032-x. Epub 2020 Jan 16.
Spinal cord injury (SCI) results in severe damage, which causes functional alterations together with loss of autonomic functions, sensations, and muscle functioning. This injury leads to apoptosis of neurons and oligodendrocytes, which further leads to dysfunction of the spinal cord due to axonal degeneration and demyelination. Taurine is non-proteogenic and an essential amino acid, which plays a major role in the growth and development of brain cells. Ascorbic acid, also known as vitamin C, is found in various foods and is known to prevent scurvy. In this study, we have investigated the therapeutic effect of ascorbic acid and taurine against SCI-induced rats. The rats were divided into the following groups: sham, control, 100 mg/kg of taurine, 100 mg/kg of ascorbic acid, and 100 mg/kg of taurine + 100 mg/kg of ascorbic acid. Treatment was continued daily for 45 consecutive days. The combined treatment of taurine and ascorbic acid decreased caspase-3, bax, pro-NGF, and p53 mRNA expression by more than 30% compared to individual treatments. The combined treatment of taurine and ascorbic acid reduced caspase-3 and p53 expression by 33.7% and 44%, respectively, compared to individual treatments. The combined treatment of taurine and ascorbic acid decreased mRNA expression of interleukin-6 (IL-6), cyclooxygenase-2, tumor necrosis factor-alpha (TNF-α), and inducible nitric oxide synthase (iNOS) compared to the individual treatments of taurine and ascorbic acid. The combined treatment of taurine and ascorbic acid also significantly recovered altered antioxidant markers, and induced lipid peroxidation to near normal levels. In summary, apoptotic, inflammatory and oxidative stress markers were significantly decreased in SCI-induced rats treated with taurine and ascorbic acid.
脊髓损伤（SCI）会导致严重损害，引发功能改变以及自主神经功能、感觉和肌肉功能丧失。这种损伤会导致神经元和少突胶质细胞凋亡，进而由于轴突变性和脱髓鞘导致脊髓功能障碍。牛磺酸是非蛋白原性必需氨基酸，在脑细胞生长发育中起主要作用。抗坏血酸，即维生素C，存在于多种食物中，已知可预防坏血病。在本研究中，我们调查了抗坏血酸和牛磺酸对脊髓损伤诱导大鼠的治疗效果。将大鼠分为以下几组：假手术组、对照组、100mg/kg牛磺酸组、100mg/kg抗坏血酸组以及100mg/kg牛磺酸 + 100mg/kg抗坏血酸组。连续45天每天进行治疗。与单独治疗相比，牛磺酸和抗坏血酸联合治疗使半胱天冬酶 - 3、bax、前神经生长因子（pro-NGF）和p53 mRNA表达降低超过30%。与单独治疗相比，牛磺酸和抗坏血酸联合治疗使半胱天冬酶 - 3和p53表达分别降低33.7%和44%。与牛磺酸和抗坏血酸单独治疗相比，牛磺酸和抗坏血酸联合治疗降低了白细胞介素 - 6（IL - 6）、环氧化酶 - 2、肿瘤坏死因子 - α（TNF - α）和诱导型一氧化氮合酶（iNOS）的mRNA表达。牛磺酸和抗坏血酸联合治疗还显著恢复了改变的抗氧化标志物，并使脂质过氧化诱导至接近正常水平。总之，在用牛磺酸和抗坏血酸治疗的脊髓损伤诱导大鼠中，凋亡、炎症和氧化应激标志物显著降低。
