Gan Yamei, Guo Liang, Gao Cong, Song Wei, Wu Jing, Liu Liming, Chen Xiulai
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, Jiangsu, China.
Sheng Wu Gong Cheng Xue Bao. 2023 Jun 25;39(6):2390-2409. doi: 10.13345/j.cjb.221008.
The use of light energy to drive carbon dioxide (CO) reduction for production of chemicals is of great significance for relieving environmental pressure and solving energy crisis. Photocapture, photoelectricity conversion and CO fixation are the key factors affecting the efficiency of photosynthesis, and thus also affect the efficiency of CO utilization. To solve the above problems, this review systematically summarizes the construction, optimization and application of light-driven hybrid system from the perspective of combining biochemistry and metabolic engineering. We introduce the latest research progress of light-driven CO reduction for biosynthesis of chemicals from three aspects: enzyme hybrid system, biological hybrid system and application of these hybrid system. In the aspect of enzyme hybrid system, many strategies were adopted such as improving enzyme catalytic activity and enhancing enzyme stability. In the aspect of biological hybrid system, many methods were used including enhancing biological light harvesting capacity, optimizing reducing power supply and improving energy regeneration. In terms of the applications, hybrid systems have been used in the production of one-carbon compounds, biofuels and biofoods. Finally, the future development direction of artificial photosynthetic system is prospected from the aspects of nanomaterials (including organic and inorganic materials) and biocatalysts (including enzymes and microorganisms).
利用光能驱动二氧化碳（CO）还原以生产化学品对于缓解环境压力和解决能源危机具有重要意义。光捕获、光电转换和CO固定是影响光合作用效率的关键因素，因此也影响CO的利用效率。为了解决上述问题，本综述从生物化学与代谢工程相结合的角度，系统总结了光驱动混合系统的构建、优化及应用。我们从酶混合系统、生物混合系统以及这些混合系统的应用三个方面介绍了光驱动CO还原用于化学品生物合成的最新研究进展。在酶混合系统方面，采用了许多策略，如提高酶催化活性和增强酶稳定性。在生物混合系统方面，使用了许多方法，包括增强生物光捕获能力、优化还原力供应和改善能量再生。在应用方面，混合系统已用于一碳化合物、生物燃料和生物食品的生产。最后，从纳米材料（包括有机和无机材料）和生物催化剂（包括酶和微生物）方面对人工光合系统的未来发展方向进行了展望。
