Liu Kang, Qiao Yangyi, Zhang Shangjie, Guo Feng, Ma Jiangfeng, Xin Fengxue, Zhang Wenming, Jiang Min
State Key Laboratory of Materials-oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210000, Jiangsu, China.
The Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, Nanjing 210000, Jiangsu, China.
Sheng Wu Gong Cheng Xue Bao. 2023 Jun 25;39(6):2430-2448. doi: 10.13345/j.cjb.221010.
Methanol has become an attractive substrate for the biomanufacturing industry due to its abundant supply and low cost. The biotransformation of methanol to value-added chemicals using microbial cell factories has the advantages of green process, mild conditions and diversified products. These advantages may expand the product chain based on methanol and alleviate the current problem of biomanufacturing, which is competing with people for food. Elucidating the pathways involving methanol oxidation, formaldehyde assimilation and dissimilation in different natural methylotrophs is essential for subsequent genetic engineering modification, and is more conducive to the construction of novel non-natural methylotrophs. This review discusses the current status of research on methanol metabolic pathways in methylotrophs, and presents recent advances and challenges in natural and synthetic methylotrophs and their applications in methanol bioconversion.
甲醇因其供应丰富且成本低廉，已成为生物制造业颇具吸引力的底物。利用微生物细胞工厂将甲醇生物转化为高附加值化学品具有绿色工艺、条件温和及产品多样等优势。这些优势可能会拓展基于甲醇的产品链，并缓解当前生物制造与人类争夺食物的问题。阐明不同天然甲基营养菌中涉及甲醇氧化、甲醛同化和异化的途径，对于后续的基因工程改造至关重要，且更有利于构建新型非天然甲基营养菌。本文综述了甲基营养菌中甲醇代谢途径的研究现状，并介绍了天然和合成甲基营养菌的最新进展与挑战及其在甲醇生物转化中的应用。
