Wang Shuxian, Fang Jiayu, Zhang Yanping, Li Yin, Zhu Taicheng
State Key Laboratory of Microbial Resources, Department of Microbial Physiological & Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Sheng Wu Gong Cheng Xue Bao. 2024 Aug 25;40(8):2747-2760. doi: 10.13345/j.cjb.240163.
Methanol has been considered one of the most important alternative carbon sources for the next-generation biomanufacturing due to its low price, mature production processes, and potential sustainability. Constructing microbial cell factories for methanol to chemical biotransformation has become a research hotspot in the green biomanufacturing industry. Focusing on the microorganisms that can naturally use methanol, we compare them with non-natural cell factories for chemical production from methanol. We discuss the key issues and challenges associated with natural cell factories for chemical production from methanol, summarize recent research progress surrounding these issues, and propose possible solutions to these challenges. This review helps to generate feasible guidelines and research strategies for the modification of natural cell factories for efficient methanol to chemical production in the future.
甲醇因其价格低廉、生产工艺成熟以及潜在的可持续性，被认为是下一代生物制造中最重要的替代碳源之一。构建用于甲醇到化学品生物转化的微生物细胞工厂已成为绿色生物制造行业的研究热点。聚焦于能够天然利用甲醇的微生物，我们将它们与用于从甲醇生产化学品的非天然细胞工厂进行比较。我们讨论了与利用甲醇进行化学品生产的天然细胞工厂相关的关键问题和挑战，总结了围绕这些问题的近期研究进展，并提出了应对这些挑战的可能解决方案。这篇综述有助于为未来改造天然细胞工厂以实现高效的甲醇到化学品生产生成可行的指导方针和研究策略。
