Zhang Jiao, Chen Jiangfeng, Chen Yixuan, Dai Lei, Dai Zhuojun
Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, Guangdong, China.
Sheng Wu Gong Cheng Xue Bao. 2023 Jun 25;39(5):2517-2545. doi: 10.13345/j.cjb.230062.
There are a large number of natural microbial communities in nature. Different populations inside the consortia expand the performance boundary of a single microbial population through communication and division of labor, reducing the overall metabolic burden and increasing the environmental adaptability. Based on engineering principles, synthetic biology designs or modifies basic functional components, gene circuits, and chassis cells to purposefully reprogram the operational processes of the living cells, achieving rich and controllable biological functions. Introducing this engineering design principle to obtain structurally well-defined synthetic microbial communities can provide ideas for theoretical studies and shed light on versatile applications. This review discussed recent progresses on synthetic microbial consortia with regard to design principles, construction methods and applications, and prospected future perspectives.
自然界中存在大量天然微生物群落。聚生体内部的不同种群通过交流与分工拓展了单个微生物种群的性能边界，降低了整体代谢负担，提高了环境适应性。基于工程原理，合成生物学设计或修改基本功能组件、基因回路和底盘细胞，以有目的地对活细胞的运作过程进行重新编程，实现丰富且可控的生物学功能。引入这一工程设计原理来获得结构明确的合成微生物群落可为理论研究提供思路，并为广泛应用带来启示。本文综述了合成微生物聚生体在设计原理、构建方法和应用方面的最新进展，并对未来前景进行了展望。
