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基于点击编码测序 v2.0 技术对氧化 DNA 损伤进行单核苷酸分辨率测序

Sequencing for oxidative DNA damage at single-nucleotide resolution with click-code-seq v2.0.

机构信息

Department of Chemistry, University of Utah, 315 S. 1400 East, Salt Lake City, UT 84112-0850, USA.

出版信息

Chem Commun (Camb). 2023 Jul 18;59(58):8997-9000. doi: 10.1039/d3cc02699j.

DOI:10.1039/d3cc02699j
PMID:37401666
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10909242/
Abstract

Oxidative damage to DNA nucleotides has many cellular outcomes that could be aided by the development of sequencing methods. Herein, the previously reported click-code-seq method for sequencing a single damage type is redeveloped to enable the sequencing of many damage types by making simple changes to the protocol (, click-code-seq v2.0).

摘要

DNA 核苷酸的氧化损伤有许多细胞后果，可以通过开发测序方法来辅助。在此，重新开发了先前报道的点击编码测序方法，以通过对方案进行简单的更改来实现多种损伤类型的测序（，点击编码测序 v2.0）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8df2/10909242/5c73c910a366/nihms-1969256-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8df2/10909242/159fc87e5dde/nihms-1969256-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8df2/10909242/232b5b532871/nihms-1969256-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8df2/10909242/5c73c910a366/nihms-1969256-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8df2/10909242/159fc87e5dde/nihms-1969256-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8df2/10909242/232b5b532871/nihms-1969256-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8df2/10909242/5c73c910a366/nihms-1969256-f0003.jpg

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J Am Chem Soc. 2022 Jan 12;144(1):454-462. doi: 10.1021/jacs.1c10490. Epub 2022 Jan 3.
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Biophys Rep (N Y). 2021 Dec 8;1(2):None. doi: 10.1016/j.bpr.2021.100017.
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