Vinogradov I V, Gablia M Yu, Rogozin D S, Tazhetdinov O H, Novikova E G, Fateev D M, Olefir Yu V, Estevesh G M
Department of Urology and Operative Nephrology with a course of oncourology, Peoples Friendship University of Russia, Moscow, Russia.
Scientific and Practical Center of Reproductive and Regenerative Medicine, Moscow, Russia.
Urologiia. 2023 May(2):73-79.
Oxidative stress considered to be the main cause of male infertility. Surgical treatment of varicocele and elimination of the inflammatory process in the male accessory glands can help reduce the level of oxidative stress, however, in most cases, antioxidant therapy is additionally prescribed. Currently, much attention is paid to regulatory peptides as a component of antioxidant therapy due to their antioxidant, anti-inflammatory and immunomodulatory properties.
To evaluate the efficiency of a complex of antimicrobial peptides and cytokines Superlymph for the treatment of male infertility associated with oxidative stress.
A total of 30 patients with elevated levels of reactive oxygen species were recruited in the open, prospective, multicenter study. Ejaculate analysis (WHO-2010), MAR-test, sperm DNA damage testing and reactive oxygen species measurement were performed. All patients received Superlymph 25 IU/day for 60 days. Antibiotics and vitamin D were also prescribed if indicated. In addition, 12 patients took dietary supplements with antioxidant action. Laboratory tests were repeated after the completion of treatment.
Superlymph therapy led to an improvement in standard semen parameters as well as decrease in sperm DNA fragmentation and oxidative stress. At the end of treatment, a significant increase in the sperm concentration was observed (46.8 [30; 87] vs. 62 [43-89], p=0.002). After treatment, there was also an increase in the median of sperm cells with normal morphology (3 [1; 7] vs. 4.5 [2; 9], p=0.002). The median sperm DNA fragmentation was lower compared to baseline, but this difference did not reach significant level (19 [14; 26] vs. 15 [10.5; 19.5], p=0.06). A significant decrease in the level of oxidative stress both in patients who took Superlymph as monotherapy (43 [27; 51] vs. 33 [22; 44], p=0.005), and as part of combination therapy with other antioxidants (31 [22; 54] vs. 21 [12; 36], p=0.009) was also observed.
Superlymph contributes to the improvement of standard ejaculate parameters as well as decrease in the level of sperm DNA fragmentation and oxidative stress.
氧化应激被认为是男性不育的主要原因。精索静脉曲张的手术治疗以及男性附属腺体炎症过程的消除有助于降低氧化应激水平，然而，在大多数情况下，还会额外开具抗氧化治疗药物。目前，由于其抗氧化、抗炎和免疫调节特性，调节肽作为抗氧化治疗的一个组成部分受到了广泛关注。
评估抗菌肽和细胞因子复合物Superlymph治疗与氧化应激相关的男性不育的疗效。
在一项开放、前瞻性、多中心研究中招募了30例活性氧水平升高的患者。进行了精液分析（WHO-2010）、MAR试验、精子DNA损伤检测和活性氧测量。所有患者接受Superlymph 25 IU/天，持续60天。如有指征，还开具了抗生素和维生素D。此外，12例患者服用了具有抗氧化作用的膳食补充剂。治疗结束后重复进行实验室检查。
Superlymph治疗导致标准精液参数得到改善，精子DNA片段化和氧化应激降低。治疗结束时，观察到精子浓度显著增加（46.8[30；87]对62[43-89]，p=0.002）。治疗后，正常形态精子细胞的中位数也有所增加（3[1；7]对4.5[2；9]，p=0.002）。精子DNA片段化中位数低于基线水平，但这种差异未达到显著水平（19[14；26]对15[10.5；19.5]，p=0.06）。在单独使用Superlymph治疗的患者（43[27；51]对33[22；44]，p=0.005）以及作为与其他抗氧化剂联合治疗一部分的患者中（31[22；54]对2l[12；36]，p=0.009），氧化应激水平均显著降低。
Superlymph有助于改善标准精液参数以及降低精子DNA片段化水平和氧化应激。
