Department of Integrated Crop Protection against Pests, Crop Research Institute, Prague, Czechia.
Department of Plant Protection, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czechia.
Pest Manag Sci. 2024 May;80(5):2342-2352. doi: 10.1002/ps.7646. Epub 2023 Jul 18.
Failures in controlling Myzus persicae by pyrethroids and carbamates have been observed in Czechia since 2018. Eleven populations collected from Czech oilseed rape fields during 2018-2021 were tested for susceptibility to 11 insecticides. The presence of a single nucleotide polymorphism (SNP) leading to knockdown resistance in M. persicae populations was screened using allelic discriminating quantitative real-time polymerase chain reaction (qPCR). The presence of mutations related with the resistance of M. persicae to pyrethroids and carbamates was detected by sequencing paratype voltage-gated sodium channel and acetylcholinesterase 2 genes, respectively.
Resistance to alpha-cypermethrin and pirimicarb was detected in most of the tested populations. The L1014F mutation was detected in 44.5% of M. persicae individuals surviving the field-recommended dose of alpha-cypermethrin. Sequencing of partial para gene for paratype voltage-gated sodium channel detected five different SNPs leading to four amino acid substitutions (kdr L1014F; s-kdr M918L; s-kdr M918T; and L932F). No pyrethroid-sensitive genotype was detected. The S431F amino acid substitution conferring resistance to carbamates was detected in 11 of 20 individuals with different pyrethroid-resistance genotypes.
Resistance of M. persicae to both pyrethroids and carbamates was detected in nine of 11 populations. High resistance of M. persicae was correlated with mutations of the sodium channel. Sulfoxaflor, flonicamid, and spirotetramat are proposed as effective compounds to control pyrethroid- and carbamate-resistant populations of M. persicae. © 2023 Society of Chemical Industry.
自 2018 年以来，捷克已观察到拟除虫菊酯和氨基甲酸酯类杀虫剂对烟粉虱的控制失败。2018 年至 2021 年期间，从捷克油菜田采集了 11 个种群，并用 11 种杀虫剂对其进行了敏感性测试。使用等位基因区分定量实时聚合酶链反应（qPCR）筛选导致烟粉虱种群击倒抗性的单个核苷酸多态性（SNP）。通过测序副型电压门控钠离子通道和乙酰胆碱酯酶 2 基因，分别检测与烟粉虱对拟除虫菊酯和氨基甲酸酯类的抗性相关的突变。
在大多数测试种群中均检测到对α-氯氰菊酯和吡虫啉的抗性。在 44.5%的幸存于田间推荐剂量的α-氯氰菊酯的烟粉虱个体中检测到 L1014F 突变。副型电压门控钠离子通道部分 para 基因测序检测到五个不同的 SNP，导致四个氨基酸取代（kdr L1014F；s-kdr M918L；s-kdr M918T；和 L932F）。未检测到对拟除虫菊酯敏感的基因型。在具有不同拟除虫菊酯抗性基因型的 20 个个体中的 11 个个体中检测到赋予对氨基甲酸酯类抗性的 S431F 氨基酸取代。
在 11 个种群中的 9 个中检测到烟粉虱对拟除虫菊酯和氨基甲酸酯类的抗性。烟粉虱的高抗性与钠离子通道的突变有关。建议使用唑虫酰胺、氟啶虫酰胺和螺虫乙酯作为控制对拟除虫菊酯和氨基甲酸酯类抗性烟粉虱种群的有效化合物。© 2023 化学工业协会。
