Department of Optical and Biophysical Systems, Institute of Physics of the Czech Academy of Sciences, Prague, 18221, Czech Republic.
Institute for Clinical and Experimental Medicine (IKEM), Prague, 14021, Czech Republic.
Sci Rep. 2023 Jul 4;13(1):10818. doi: 10.1038/s41598-023-38015-x.
Dramatically increased levels of electromagnetic radiation in the environment have raised concerns over the potential health hazards of electromagnetic fields. Various biological effects of magnetic fields have been proposed. Despite decades of intensive research, the molecular mechanisms procuring cellular responses remain largely unknown. The current literature is conflicting with regards to evidence that magnetic fields affect functionality directly at the cellular level. Therefore, a search for potential direct cellular effects of magnetic fields represents a cornerstone that may propose an explanation for potential health hazards associated with magnetic fields. It has been proposed that autofluorescence of HeLa cells is magnetic field sensitive, relying on single-cell imaging kinetic measurements. Here, we investigate the magnetic field sensitivity of an endogenous autofluorescence in HeLa cells. Under the experimental conditions used, magnetic field sensitivity of an endogenous autofluorescence was not observed in HeLa cells. We present a number of arguments indicating why this is the case in the analysis of magnetic field effects based on the imaging of cellular autofluorescence decay. Our work indicates that new methods are required to elucidate the effects of magnetic fields at the cellular level.
环境中电磁辐射水平的急剧增加引起了人们对电磁场潜在健康危害的关注。已经提出了磁场的各种生物效应。尽管经过了数十年的密集研究，但仍不清楚获取细胞反应的分子机制。目前的文献在磁场是否直接影响细胞水平的功能方面存在矛盾。因此，寻找磁场对细胞的潜在直接影响是一个基石，可能为与磁场相关的潜在健康危害提供解释。已经提出，依赖于单细胞成像动力学测量，HeLa 细胞的自发荧光对磁场敏感。在这里，我们研究了 HeLa 细胞中内源性自发荧光的磁场敏感性。在使用的实验条件下，在 HeLa 细胞中未观察到内源性自发荧光的磁场敏感性。我们提出了一些论据，为什么在基于细胞自发荧光衰减的成像的磁场效应分析中就是这种情况。我们的工作表明，需要新的方法来阐明磁场在细胞水平上的作用。
