采用高效液相色谱法从毛花洋地黄中分离并定量测定某些强心苷。

Isolation and quantitative determination of some cardioactive glycosides from Digitalis lanata by high-performance liquid chromatography.

作者信息

Orosz F, Nuridsány M, Ovádi J

出版信息

Anal Biochem. 1986 Jul;156(1):171-5. doi: 10.1016/0003-2697(86)90169-7.

Abstract

A rapid extraction method followed by high-performance liquid chromatographic assay was developed for the quantitative determination of the cardioactive glycosides of Digitalis lanata. The leaf samples were extracted with water or aqueous alcohols. The simple extraction method gives a better yield than the methods described previously. Lanatoside C and its metabolites have been separated on a reversed-phase column with various mixtures of acetonitrile, methanol, and water as mobile phases for isocratic elution. Extraction and quantitative determination of lanatoside C and digoxin from a leaf sample require not more than 30 min.

摘要

建立了一种快速提取方法，随后采用高效液相色谱法测定毛花洋地黄中的强心苷。叶片样品用水或含水醇进行提取。这种简单的提取方法比先前描述的方法产率更高。洋地黄苷C及其代谢产物已在反相柱上分离，以乙腈、甲醇和水的各种混合物作为流动相进行等度洗脱。从叶片样品中提取并定量测定洋地黄苷C和地高辛所需时间不超过30分钟。