Choi H O, Hwang K J
Anal Biochem. 1986 Jul;156(1):176-81. doi: 10.1016/0003-2697(86)90170-3.
Lipophilic chelates such as 8-hydroxyquinoline, acetylacetone, and tropolone are useful to load high levels of radioactive cations into the inner aqueous compartments of liposomes for investigating the fate of liposomes by the technique of gamma imaging or gamma-ray perturbed angular correlation measurements. However, if lipophilic chelates are not completely removed from liposomes the very same lipophilic chelates can also cause leakage of the entrapped cations from liposomes. Thus, it is essential to make sure that all the lipophilic chelates are removed from liposomes after the loading process. The results of the present study show that more than 99.85% of acetylacetone in liposomal suspension can be removed by a minicolumn of AG1-X8 (phosphate form) anion exchange resin. Virtually all the 8-hydroxyquinoline and tropolone in liposomal suspension are adsorbed tightly to the resin. The procedure is rapid, and the dilution of liposomes is minimal. For experiments involving high levels of gamma-emitting radionuclides, the cleaning up process of removing lipophilic chelates from liposomes can be conveniently operated behind a lead glass.
亲脂性螯合剂，如8-羟基喹啉、乙酰丙酮和托酚酮，可用于将高浓度放射性阳离子载入脂质体的内部水相区室，以便通过γ成像技术或γ射线扰动角关联测量来研究脂质体的命运。然而，如果亲脂性螯合剂没有从脂质体中完全去除，那么同样的亲脂性螯合剂也会导致被包裹的阳离子从脂质体中泄漏。因此，在加载过程之后确保所有亲脂性螯合剂都从脂质体中去除至关重要。本研究结果表明，脂质体悬浮液中超过99.85%的乙酰丙酮可以通过AG1-X8（磷酸盐形式）阴离子交换树脂的微型柱去除。脂质体悬浮液中几乎所有的8-羟基喹啉和托酚酮都被紧密吸附到树脂上。该过程快速，脂质体的稀释最小。对于涉及高浓度γ发射放射性核素的实验，从脂质体中去除亲脂性螯合剂的清理过程可以在铅玻璃后方便地进行。
