Keler T, Nowotny A
Anal Biochem. 1986 Jul;156(1):189-93. doi: 10.1016/0003-2697(86)90172-7.
A metachromatic dye, 1,9-dimethylmethylene blue (DMB) reacts with bacterial endotoxins. This interaction results in a shift of the absorption maximum of DMB to shorter wavelengths. The findings indicate that the negatively charged lipid moiety of the endotoxic lipopolysaccharide reacts with DMB. The lowest amount of endotoxin detectable by the procedure described here is approximately one microgram. The dye could not be used in the presence of serum components. DMB mixed to column chromatographic effluent showed good resolution in continuous monitoring of endotoxin components leaving the column.
一种异染染料，1,9-二甲基亚甲蓝（DMB）可与细菌内毒素发生反应。这种相互作用导致DMB的最大吸收波长向更短波长偏移。研究结果表明，内毒素脂多糖带负电荷的脂质部分与DMB发生反应。此处所述方法可检测到的内毒素最低量约为1微克。该染料不能在血清成分存在的情况下使用。在连续监测从柱中流出的内毒素成分时，与柱色谱流出物混合的DMB显示出良好的分辨率。
