Department of Family Medicine and Therapy, Poltava State Medical University, Poltava, Ukraine.
Chair of Internal Medicine and Gerontology, Shupyk National University of Public Health of Ukraine, Kyiv, Ukraine.
Turk J Gastroenterol. 2023 Aug;34(8):822-830. doi: 10.5152/tjg.2023.21985.
The number of cases of irritable bowel syndrome is growing worldwide, in which adenomatous polyps can develop as a result of microinflammation of the colonic epithelium. Our study was aimed at the identification of the possible effect of single-nucleotide polymorphisms on the risk of the development of irritable bowel syndrome-related colonic adenomatous polyps.
The study involved 187 irritable bowel syndrome patients. The single-nucleotide polymorphisms were investigated by the polymerase chain reaction method and DNA was extracted with the phenol-chloroform: interleukin-1β gene-31C/T (rs1143627), -511C/T (rs16944); interleukin-6 gene-174G/C (rs1800795); interleukin-10 gene-592C/A (rs1800872), -819T/C (rs1800871), -1082A/G (rs1800896); Toll-like receptor-2 gene Arg753Gln (rs5743708); Toll-like receptor-4 gene Thr399ile (rs4986791), Asp299Gly (rs4986790); and metalloproteinase-9 gene-8202A/G (rs11697325). The study of polymorphic loci was checked for compliance with the Hardy- Weinberg equilibrium using Fisher's exact test along with the analyses of the frequency of alleles and the genotypes.
The association of diseases with G allele Toll-like receptor-2 gene Arg753Gln (rs5743708) was revealed in irritable bowel syndrome patients with adenomatous polyps of the colon (P < .0006) and AG single-nucleotide polymorphisms s of Toll-like receptor-2 gene (χ2 = 12.78, P < .002); A allele had a protective effect. The AG genotype metalloproteinase-9 gene-8202A/G (rs11697325) polymorphism in irritable bowel syndrome patients with adenomatous polyps of the colon had a protective effect (P < .05). AA genotype interleukin-10 gene-1082A/G (rs1800896) polymorphism in the irritable bowel syndrome patient (χ2 = 33.97, 4.0E-8) can be considered as the risk for adenomatous polyps of the colon in irritable bowel syndrome.
G allele Toll-like receptor-2 gene Arg753Gln (rs5743708) and AA genotype interleukin-10 gene-1082A/G (rs1800896) polymorphisms can be the marker of the emergence of adenomatous polyps of the colon concomitant with irritable bowel syndrome.
全世界范围内肠易激综合征的病例数量正在增加，其中结肠上皮的微炎症可能导致腺瘤性息肉的形成。我们的研究旨在确定单核苷酸多态性对肠易激综合征相关结肠腺瘤性息肉发展风险的可能影响。
本研究纳入了 187 例肠易激综合征患者。通过聚合酶链反应方法检测单核苷酸多态性，并使用苯酚-氯仿法提取 DNA。研究的单核苷酸多态性包括白细胞介素-1β基因-31C/T（rs1143627）、-511C/T（rs16944）；白细胞介素-6 基因-174G/C（rs1800795）；白细胞介素-10 基因-592C/A（rs1800872）、-819T/C（rs1800871）、-1082A/G（rs1800896）；Toll 样受体-2 基因 Arg753Gln（rs5743708）；Toll 样受体-4 基因 Thr399ile（rs4986791）、Asp299Gly（rs4986790）；以及金属蛋白酶-9 基因-8202A/G（rs11697325）。使用 Fisher 精确检验检查多态性位点与 Hardy-Weinberg 平衡的一致性，并分析等位基因和基因型的频率。
在患有结肠腺瘤性息肉的肠易激综合征患者中，Toll 样受体-2 基因 Arg753Gln（rs5743708）的 G 等位基因与疾病相关（P <.0006），并且 Toll 样受体-2 基因的 AG 单核苷酸多态性（χ2 = 12.78，P <.002）；A 等位基因具有保护作用。在患有结肠腺瘤性息肉的肠易激综合征患者中，金属蛋白酶-9 基因-8202A/G（rs11697325）的 AG 基因型具有保护作用（P <.05）。在肠易激综合征患者中，白细胞介素-10 基因-1082A/G（rs1800896）的 AA 基因型具有保护作用（χ2 = 33.97，4.0E-8），可被认为是肠易激综合征患者发生结肠腺瘤性息肉的风险因素。
Toll 样受体-2 基因 Arg753Gln（rs5743708）的 G 等位基因和白细胞介素-10 基因-1082A/G（rs1800896）的 AA 基因型可能是肠易激综合征伴发结肠腺瘤性息肉的标志物。
