肠易激综合征中的白细胞介素-8 和 -10 基因多态性。
Interleukin-8 and -10 gene polymorphisms in irritable bowel syndrome.
机构信息
Dirección de Investigación, Hospital General "Dr. Manuel Gea González", Calzada de Tlalpan 4800, Colonia Sección XVI, 14080 Mexico, DF, Mexico.
出版信息
Mol Biol Rep. 2012 Sep;39(9):8837-43. doi: 10.1007/s11033-012-1745-2. Epub 2012 Jun 28.
Irritable bowel syndrome (IBS) is one of the most common gastrointestinal diagnoses seen by primary care providers and gastroenterologists. Proinflammatory cytokines interleukin (IL)-6 and IL-8 have been found increased in IBS patients. Cytokine gene single nucleotide polymorphisms (SNPs) of IL-8 and IL-10 have not been assessed in Mexican IBS patients. DNA was extracted from peripheral blood leucocytes of 45 IBS unrelated patients and 137 controls. Allele, genotype, and haplotype frequencies were determined by analyzing SNPs of IL-8 and IL-10 genes. IL-8 + 396 G allele (P = 0.02), IL-8 + 396(G/G) and IL-8 + 781(C/T) genotypes (P < 0.001), IL-10 - 1082A allele and IL-10 - 1082(A/A) genotype (P < 0.001) were significantly increased in the IBS group. Haplotypes IL-8 ATCC (P = 0.03) and IL-10 ACC (P < 0.001) were associated with susceptibility to develop IBS. An association of certain polymorphisms of IL-8 and IL-10 in IBS patients compared to controls was demonstrated, suggesting a role of these cytokine SNPs in the pathophysiology of IBS.
肠易激综合征（IBS）是初级保健提供者和胃肠病学家最常见的胃肠道诊断之一。研究发现，IBS 患者的促炎细胞因子白细胞介素（IL）-6 和 IL-8 增加。尚未评估墨西哥 IBS 患者的 IL-8 和 IL-10 细胞因子基因单核苷酸多态性（SNP）。从 45 例 IBS 无关患者和 137 例对照者的外周血白细胞中提取 DNA。通过分析 IL-8 和 IL-10 基因的 SNP 来确定等位基因、基因型和单倍型频率。IL-8 + 396 G 等位基因（P = 0.02）、IL-8 + 396（G/G）和 IL-8 + 781（C/T）基因型（P < 0.001）、IL-10 - 1082A 等位基因和 IL-10 - 1082（A/A）基因型（P < 0.001）在 IBS 组中显著增加。IL-8 ATCC 单倍型（P = 0.03）和 IL-10 ACC 单倍型（P < 0.001）与 IBS 的易感性相关。与对照组相比，IBS 患者中某些 IL-8 和 IL-10 多态性的关联表明这些细胞因子 SNP 在 IBS 的病理生理学中起作用。
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