用聚丙烯酰胺-琼脂糖复合凝胶对35S标记的蛋白聚糖进行电泳，并通过荧光自显影对其进行可视化。
Electrophoresis of 35S-labeled proteoglycans on polyacrylamide-agarose composite gels and their visualization by fluorography.
作者信息
Carney S L, Bayliss M T, Collier J M, Muir H
出版信息
Anal Biochem. 1986 Jul;156(1):38-44. doi: 10.1016/0003-2697(86)90150-8.
PMID:3740416
Abstract
We have developed techniques for the electrophoresis of 35S-labeled proteoglycans on polyacrylamide-agarose gel slabs and subsequent fixation, impregnation, and fluorography of such electrophoretograms. The procedure permits the examination of newly synthesized proteoglycan subspecies using a rapid technique, previously unavailable for these labeled molecules.
摘要
我们已经开发出了在聚丙烯酰胺 - 琼脂糖凝胶板上对35S标记的蛋白聚糖进行电泳，以及随后对此类电泳图谱进行固定、浸渍和荧光显影的技术。该程序允许使用一种快速技术来检测新合成的蛋白聚糖亚类，而这种技术以前对于这些标记分子是不可用的。
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