Heinegård D, Sommarin Y, Hedbom E, Wieslander J, Larsson B
Anal Biochem. 1985 Nov 15;151(1):41-8. doi: 10.1016/0003-2697(85)90050-8.
The agarose-polyacrylamide gel electrophoresis procedure for the analysis of proteoglycans originally described by C. A. McDevitt and H. Muir (1971, Anal. Biochem. 44, 612-622) has been modified to minimize trailing and to allow the analysis of crude samples, i.e., tissue extracts. A slab gel system was used, permitting reproducible analysis of many samples. Procedures are described that can be used to separate and quantify several subpopulations of proteoglycans and also to quantify the proportion of proteoglycans capable of aggregating with hyaluronic acid. Applications of the procedure include transfer to nitrocellulose paper followed by immunological detection of proteoglycans as well as fluorography of separated, radiolabeled proteoglycans.
最初由C. A. 麦克德维特和H. 缪尔（1971年，《分析生物化学》44卷，612 - 622页）描述的用于蛋白聚糖分析的琼脂糖 - 聚丙烯酰胺凝胶电泳程序已被改进，以尽量减少拖尾现象，并允许对粗样品（即组织提取物）进行分析。使用了平板凝胶系统，可对多个样品进行可重复分析。文中描述了可用于分离和定量蛋白聚糖的几个亚群以及定量能够与透明质酸聚集的蛋白聚糖比例的程序。该程序的应用包括转移到硝酸纤维素纸上，随后对蛋白聚糖进行免疫检测以及对分离的放射性标记蛋白聚糖进行荧光显影。
