Laboratory of Plant Breeding and Biometry, Department of Crop Science, Agricultural University of Athens, Athens, 11855, Greece.
Molecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.
Planta. 2023 Jul 5;258(2):37. doi: 10.1007/s00425-023-04182-4.
Nicotiana tabacum exhibits recovery response towards tomato leaf curl Gujarat virus. Transcriptome analysis revealed the differential expression of defense-related genes. Genes encoding for cysteine protease inhibitor, hormonal- and stress-related to DNA repair mechanism are found to be involved in the recovery process. Elucidating the role of host factors in response to viral infection is crucial in understanding the plant host-virus interaction. Begomovirus, a genus in the family Geminiviridae, is reported throughout the globe and is known to cause serious crop diseases. Tomato leaf curl Gujarat virus (ToLCGV) infection in Nicotiana tabacum resulted in initial symptom expression followed by a quick recovery in the systemic leaves. Transcriptome analysis using next-generation sequencing (NGS) revealed a large number of differentially expressed genes both in symptomatic as well as recovered leaves when compared to mock-inoculated plants. The virus infected N. tabacum results in alteration of various metabolic pathways, phytohormone signaling pathway, defense related protein, protease inhibitor, and DNA repair pathway. RT-qPCR results indicated that Germin-like protein subfamily T member 2 (NtGLPST), Cysteine protease inhibitor 1-like (NtCPI), Thaumatin-like protein (NtTLP), Kirola-like (NtKL), and Ethylene-responsive transcription factor ERF109-like (NtERTFL) were down-regulated in symptomatic leaves when compared to recovered leaves of ToLCGV-infected plants. In contrast, the Auxin-responsive protein SAUR71-like (NtARPSL) was found to be differentially down-regulated in recovered leaves when compared to symptomatic leaves and the mock-inoculated plants. Lastly, Histone 2X protein like (NtHH2L) gene was found to be down-regulated, whereas Uncharacterized (NtUNCD) was up-regulated in both symptomatic as well as recovered leaves compared to the mock-inoculated plants. Taken together, the present study suggests potential roles of the differentially expressed genes that might govern tobacco's susceptibility and/or recovery response towards ToLCGV infection.
烟草对番茄曲叶病毒表现出恢复反应。转录组分析显示防御相关基因的差异表达。发现参与恢复过程的基因编码半胱氨酸蛋白酶抑制剂、激素和与 DNA 修复机制相关的应激。阐明宿主因子在病毒感染中的作用对于理解植物宿主-病毒相互作用至关重要。双生病毒是双生病毒科的一个属，分布于全球，已知会导致严重的作物病害。番茄曲叶病毒（ToLCGV）感染烟草后，系统叶片会先出现初始症状，然后迅速恢复。使用下一代测序（NGS）进行转录组分析显示，与 mock 接种植物相比，在有症状和恢复的叶片中都有大量差异表达的基因。病毒感染烟草会改变各种代谢途径、植物激素信号通路、防御相关蛋白、蛋白酶抑制剂和 DNA 修复途径。RT-qPCR 结果表明，与恢复的 ToLCGV 感染植物的叶片相比，在有症状的叶片中，类胚乳蛋白亚家族 T 成员 2（NtGLPST）、半胱氨酸蛋白酶抑制剂 1 样（NtCPI）、类硫素蛋白（NtTLP）、Kirola 样（NtKL）和乙烯响应转录因子 ERF109 样（NtERTFL）下调。相比之下，在恢复的叶片中，Auxin-responsive protein SAUR71-like（NtARPSL）的表达与有症状的叶片和 mock 接种的植物相比差异下调。最后，发现组蛋白 2X 蛋白样（NtHH2L）基因下调，而未鉴定（NtUNCD）在有症状和恢复的叶片中均上调，与 mock 接种的植物相比。综上所述，本研究表明差异表达基因可能在烟草对 ToLCGV 感染的易感性和/或恢复反应中发挥潜在作用。
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