利用牛免疫结合酵母表面展示技术分离抗原特异性非常规牛超长互补决定区3重链抗体
Isolation of Antigen-Specific Unconventional Bovine Ultra-Long CDR3H Antibodies Using Cattle Immunization in Combination with Yeast Surface Display.
作者信息
Arras Paul, Zimmermann Jasmin, Lipinski Britta, Yanakieva Desislava, Klewinghaus Daniel, Krah Simon, Kolmar Harald, Pekar Lukas, Zielonka Stefan
机构信息
Antibody Discovery and Protein Engineering, Merck Healthcare KGaA, Darmstadt, Germany.
Institute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, Darmstadt, Germany.
出版信息
Methods Mol Biol. 2023;2681:113-129. doi: 10.1007/978-1-0716-3279-6_8.
Cattle are known for their repertoire of antibodies harboring extremely long CDR3H regions that form extensive "knob on stalk" cysteine-rich structures. The compact knob domain allows for the recognition of epitopes potentially not accessible to classical antibodies. To effectively access the potential of bovine-derived antigen-specific ultra-long CDR3 antibodies, a straightforward and effective high-throughput method based on yeast surface display and fluorescence-activated cell sorting is described.
牛以其拥有极长互补决定区3重链（CDR3H）区域的抗体库而闻名，这些区域形成广泛的“柄上瘤”富含半胱氨酸的结构。紧密的瘤结构域能够识别经典抗体可能无法触及的表位。为了有效利用源自牛的抗原特异性超长CDR3抗体的潜力，本文描述了一种基于酵母表面展示和荧光激活细胞分选的直接有效的高通量方法。
Zotero 插件