Lambré C R, le Maho S, di Bella G, de Cremoux H, Atassi K, Bignon J
Am Rev Respir Dis. 1986 Aug;134(2):238-42. doi: 10.1164/arrd.1986.134.2.238.
In this work, using bronchoalveolar lavage fluids (BALF), we demonstrated the presence of complement within airways by assaying hemolytic activity of the whole classical pathway (CH50) and by measuring the complement component C2 (C2H50). Patients with sarcoidosis, patients with idiopathic pulmonary fibrosis (IPF), and healthy control subjects were compared. No CH50 activity was found in BALF from healthy control subjects (n = 9), but some activity (mean, 20 CH50) was associated with IPF (n = 7). Complement activities ranged from 40 to 554 CH50 in patients with sarcoidosis (n = 27). During the treatment, complement activity decreased in BALF from the few patients in our series who received corticotherapy. C2 hemolytic activity was detected in BALF from the normal control group (in the absence of CH50 activity). In the sarcoidosis and IPF groups when CH50 was present, the variations in the C2/CH50 ratio were studied. The high ratio observed in BALF from patients with sarcoidosis and a chronic derangement of alveolar structure suggests either an increased C2 production or an alternative complement pathway (C2-independent) activation within their lungs.
在本研究中，我们使用支气管肺泡灌洗液（BALF），通过检测整个经典途径的溶血活性（CH50）以及测量补体成分C2（C2H50），来证明气道内补体的存在。我们比较了结节病患者、特发性肺纤维化（IPF）患者和健康对照者。在健康对照者（n = 9）的BALF中未发现CH50活性，但在IPF患者（n = 7）中发现了一些活性（平均为20 CH50）。结节病患者（n = 27）的补体活性范围为40至554 CH50。在治疗期间，我们系列中少数接受皮质激素治疗的患者的BALF中的补体活性降低。在正常对照组的BALF中检测到了C2溶血活性（在没有CH50活性的情况下）。在存在CH50的结节病和IPF组中，研究了C2/CH50比值的变化。在结节病患者的BALF中观察到的高比值以及肺泡结构的慢性紊乱表明，其肺部要么C2产生增加，要么替代补体途径（不依赖C2）被激活。
