Faculty of Environment, Jan Evangelista Purkyně University, Pasteurova 3632/15, 40096, Ústí nad Labem, Czech Republic.
Faculty of Environment, Jan Evangelista Purkyně University, Pasteurova 3632/15, 40096, Ústí nad Labem, Czech Republic.
J Environ Manage. 2023 Oct 15;344:118475. doi: 10.1016/j.jenvman.2023.118475. Epub 2023 Jul 3.
Second generation biofuel crop Miscanthus x giganteus (Mxg) was studied as a candidate for petroleum hydrocarbons (PHs) contaminated soil phytomanagement. The soil was polluted by diesel in wide concentration gradient up to 50 g⋅kg in an ex-situ pot experiment. The contaminated soil/plant interactions were investigated using plant biometric and physiological parameters, soil physico-chemical and microbial community's characteristics. The plant parameters and chlorophyll fluorescence indicators showed an inhibitory effect of diesel contamination; however much lower than expected from previously published results. Moreover, lower PHs concentrations (5 and 10 g⋅kg) resulted in positive reinforcement of electron transport as a result of hormesis effect. The soil pH did not change significantly during the vegetation season. The decrease of total organic carbon was significantly lower in planted pots. Soil respiration and dehydrogenases activity increased with the increasing contamination indicating ongoing PHs biodegradation. In addition, microbial biomass estimated by phospholipid fatty acids increased only at higher PHs concentrations. Higher dehydrogenases values were obtained in planted pots compared to unplanted. PHs degradation followed the first-order kinetics and for the middle range of contamination (10-40 g⋅kg) significantly lower PHs half-lives were determined in planted than unplanted soil pointing on phytoremediation. Diesel degradation was in range 35-70 % according to pot variant. Results confirmed the potential of Mxg for diesel contaminated soils phytomanagement mainly in PHs concentrations up to 20 g⋅kg where phytoremediation was proved, and biomass yield was reduced only by 29 %.
第二代生物燃料作物芒属巨芒（Mxg）被研究为石油烃（PHs）污染土壤植物修复的候选植物。在一项异位盆栽实验中，土壤被柴油以宽浓度梯度（高达 50 g·kg）污染。通过植物生物计量和生理参数、土壤理化特性和微生物群落特征，研究了污染土壤/植物的相互作用。植物参数和叶绿素荧光指标显示柴油污染存在抑制作用；然而，其抑制作用比以前发表的结果预期的要低得多。此外，较低的 PHs 浓度（5 和 10 g·kg）由于应激效应导致电子传递产生正强化。在植被季节，土壤 pH 值没有明显变化。总有机碳的减少在种植盆中显著较低。随着污染的增加，土壤呼吸和脱氢酶活性增加，表明 PHs 生物降解正在进行。此外，通过磷脂脂肪酸估计的微生物生物量仅在较高的 PHs 浓度下增加。与未种植的盆相比，种植的盆中获得了更高的脱氢酶值。PHs 降解遵循一级动力学，在污染的中范围（10-40 g·kg），种植土壤中的 PHs 半衰期明显低于未种植土壤，表明存在植物修复。根据盆的变化，柴油降解率在 35-70%之间。结果证实了 Mxg 对 PHs 浓度高达 20 g·kg 的柴油污染土壤植物修复的潜力，在该浓度下证明了植物修复，生物量仅减少了 29%。
