King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Jeddah, Saudi Arabia.
King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Jeddah, Saudi Arabia; Department of Pathology and Laboratory Medicine, Ministry of the National Guard - Health Affairs, Jeddah, Saudi Arabia.
Stem Cell Res. 2023 Sep;71:103158. doi: 10.1016/j.scr.2023.103158. Epub 2023 Jun 25.
Myoglobin (MB) is a cytoplasmic hemoprotein that is predominantly expressed in the heart and oxidative myofibers of skeletal muscle. It has been demonstrated that MB binds to oxygen and promotes its diffusion for energy production in the mitochondria. Recently, MB was found to be expressed in different forms of malignant tumors and cancer cell lines. Further studies using gene disruption technology will enhance the understanding of MB's role in human cardiovascular biology and cancers. Here, we describe the generation of a homozygous MB knockout in human embryonic stem cells (hESC-MB) via CRISPR/Cas9 to study MB function in human biology and diseases.
肌红蛋白（MB）是一种主要在心脏和骨骼肌的氧化肌纤维中表达的细胞质血红素蛋白。已经证明，MB 结合氧气并促进其在线粒体中扩散以产生能量。最近，MB 被发现存在于不同形式的恶性肿瘤和癌细胞系中。使用基因敲除技术的进一步研究将增强对 MB 在人类心血管生物学和癌症中的作用的理解。在这里，我们通过 CRISPR/Cas9 技术在人胚胎干细胞（hESC-MB）中生成了纯合的 MB 敲除，以研究 MB 在人类生物学和疾病中的功能。
